Department of Medical Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Iran J Med Sci. 2024 Feb 1;49(2):121-129. doi: 10.30476/IJMS.2023.96413.2795. eCollection 2024 Feb.
Stem cell-derived secretome (SE) released into the extracellular space contributes to tissue repair. The present study aimed to investigate the impact of isolated secretome (SE) from adipose-derived mesenchymal stem cells (ASCs) on () lesions in BALB/c mice.
This experimental study was conducted at Ahvaz University of Medical Sciences (Ahvaz, Iran) in 2021. Forty female BALB/c mice were infected with stationary phase promastigotes through intradermal injection in the bottom of their tail and randomly divided into four groups (n=10 per group). The mice were given SE (20 mg/mL), either alone or in combination with Glucantime (GC, 20 mg/mL/Kg), meglumine antimoniate (20 mg/mL/Kg) for the GC group, and phosphate-buffered saline (PBS) for the control group. After eight weeks, the lesion size, histopathology, the levels of Interleukin 10 (IL-10), and Interleukin 12 (IL-12) were assessed. For the comparison of values between groups, the parametric one-way ANOVA was used to assess statistical significance.
At the end of the experiment, the mice that received SE had smaller lesions (4.56±0.83 mm versus 3.62±0.59 mm, P=0.092), lower levels of IL-10 (66.5±9.7 pg/mL versus 285.4±25.2 pg/mL, P<0.001), and higher levels of IL-12 (152.2±14.2 pg/mL versus 24.2±4.4 pg/mL, P<0.001) than the control. Histopathology findings revealed that mice treated with SE had a lower parasite burden in lesions and spleen than the control group.
The current study demonstrated that ADSC-derived SE could protect mice infected with against leishmaniasis.
干细胞衍生的细胞外泌体（SE）释放到细胞外空间有助于组织修复。本研究旨在探讨分离的脂肪间充质干细胞（ASCs）分泌的 SE 对 BALB/c 小鼠 () 病变的影响。
本实验研究于 2021 年在伊朗阿瓦兹医科大学进行。40 只雌性 BALB/c 小鼠通过尾底部皮内注射感染静止期前鞭毛体，随机分为 4 组（每组 10 只）。分别给予 SE（20mg/mL），单独或与葡萄糖酸锑钠（GC，20mg/mL/Kg）、葡甲胺锑（20mg/mL/Kg）联合用药（GC 组），磷酸盐缓冲液（PBS）对照组。8 周后，评估病变大小、组织病理学、白细胞介素 10（IL-10）和白细胞介素 12（IL-12）水平。为了比较组间值，采用参数单向方差分析评估统计学意义。
实验结束时，接受 SE 治疗的小鼠病变较小（4.56±0.83mm 比 3.62±0.59mm，P=0.092），IL-10 水平较低（66.5±9.7pg/mL 比 285.4±25.2pg/mL，P<0.001），IL-12 水平较高（152.2±14.2pg/mL 比 24.2±4.4pg/mL，P<0.001），与对照组相比。组织病理学检查结果显示，与对照组相比，SE 治疗组小鼠病变和脾脏中的寄生虫负荷较低。
本研究表明，ADSC 衍生的 SE 可保护感染的 BALB/c 小鼠免受利什曼病的侵害。
