Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing 210098, China.
Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing 210098, China.
Bioresour Technol. 2021 Mar;323:124574. doi: 10.1016/j.biortech.2020.124574. Epub 2021 Jan 4.
This study investigated the fate of ciprofloxacin (CIP) in wetlands dominated by Vallisneria spiralis. About 99% of CIP was degraded from overlaying water within 4 days of treatment but significantly inhibited the nutrient removal capacity (TN, TP, and COD) by causing a drastic reduction in microbial aggregation in epiphytic biofilm and bacterial biodiversity. CIP triggered resistance mechanisms among dominant bacteria phyla such as Proteobacteria, Actinobacteria, and Planctomycetes causing their increased relative abundance. Additionally, the relative abundances of eukaryotic microorganisms (including; Chloroplastida, Metazoa, and Rhizaria) and 13 ARGs subtypes (including; Efflux pump, Tetracycline, Multi-drug, Rifampin, Beta-lactam, Peptide, Trimethoprim) were significantly increased. While dominant metabolic pathways such as Carbohydrate, amino acid, energy and nucleotide metabolism were inhibited. This study revealed that V. spiralis has great sorption capacity for CIP than sediment and though CIP was effectively removed from the overlying water, it caused a prolonged effect on the epiphytic biofilm microbial communities.
本研究调查了水蕴草湿地中环丙沙星（CIP）的归宿。在处理后的 4 天内，约 99%的 CIP 从覆盖水中降解，但由于显著降低了附生生物膜中的微生物聚集，从而显著抑制了养分去除能力（TN、TP 和 COD）。CIP 引发了优势细菌门（如变形菌门、放线菌门和浮霉菌门）中的抗性机制，导致其相对丰度增加。此外，真核微生物（包括叶绿体、原生动物和根肿菌门）和 13 种 ARG 亚型（包括外排泵、四环素、多药、利福平、β-内酰胺、肽、甲氧苄啶）的相对丰度也显著增加。同时，碳水化合物、氨基酸、能量和核苷酸代谢等主要代谢途径受到抑制。本研究表明，水蕴草对 CIP 的吸附能力大于沉积物，虽然 CIP 从覆盖水中有效去除，但它对附生生物膜微生物群落产生了持久的影响。
