Dai Qi, Liu Rui, Liang Yu-Ting, Shu Xiao-Ming, Xu Can-Can, Chen Lü-Jun
Zhejiang Provincial Key Laboratory of Water Science and Technology, Department of Environment in Yangtze Delta Region Institute of Tsinghua University, Zhejiang, Jiaxing 314006, China.
Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Huan Jing Ke Xue. 2018 Mar 8;39(3):1333-1341. doi: 10.13227/j.hjkx.201706179.
A membrane bioreactor (MBR) was used to treat ciprofloxacin (CIP)-contaminated artificial wastewater. The microbial community structure and the abundance of antibiotic resistant genes (ARGs) in the MBR were studied at four CIP dosages (0, 5 mg·L, 10 mg·L, and 15 mg·L). The results showed that Proteobacteria and Bacteroidetes remained the dominant phylum, with relative abundances of 57.5% and 12.7%, respectively, as the dosage of CIP was increased from 0 mg·L to 15 mg·L. Rhodocyclaceae, Chitinophagaceae, and Comamonadaceae became the dominant family with abundances of 29.96%, 5.44%, and 6.60%, respectively. , and became the dominant genus, with relative abundances of 21.70%, 7.56%, 5.24%, and 4.15%, respectively. The decrease of Chao1, ACE, and Shannon and the increase of Simpson indicated a decrease in microbial abundance and diversity. The relative abundances of , and decreased, which caused a decrease in the NH-N removal rate. A CIP-ARGs analysis revealed that the relative abundances of , and were increased, beginning after the sludge was dosed with 5 mg·Lof CIP for 33 days, which augmented the risk for microbial drug-resistance.
采用膜生物反应器（MBR）处理受环丙沙星（CIP）污染的人工废水。在四种CIP剂量（0、5mg·L、10mg·L和15mg·L）下研究了MBR中的微生物群落结构和抗生素抗性基因（ARG）丰度。结果表明，随着CIP剂量从0mg·L增加到15mg·L，变形菌门和拟杆菌门仍然是优势菌门，相对丰度分别为57.5%和12.7%。红环菌科、噬几丁质菌科和丛毛单胞菌科成为优势科，丰度分别为29.96%、5.44%和6.60%。 以及 成为优势属，相对丰度分别为21.70%、7.56%、5.24%和4.15%。Chao1、ACE和Shannon指数的降低以及Simpson指数的升高表明微生物丰度和多样性降低。 、 以及 的相对丰度下降，导致NH-N去除率降低。对CIP-ARGs的分析表明，在污泥投加5mg·L CIP 33天后， 、 以及 的相对丰度开始增加，这增加了微生物耐药性的风险。
