蓝狐（北极狐）附睾中β-防御素108表达量下降与精子活力降低有关。
Declined expressing mRNA of beta-defensin 108 from epididymis is associated with decreased sperm motility in blue fox (Vulpes lagopus).
作者信息
Wu Ping, Liu Tao-Lin, Li Ling-Ling, Liu Zhi-Ping, Tian Li-Hong, Hou Zhi-Jun
机构信息
College of Wildlife and Protected Area, Northeast Forestry University, Harbin, China.
出版信息
BMC Vet Res. 2021 Jan 7;17(1):12. doi: 10.1186/s12917-020-02697-6.
BACKGROUND
Fecundity is important for farm blue fox (Vulpes lagopus), who with asthenospermia have be a problem in some of farms in China. A key symptom of asthenospermia is decreased sperm motility. The decreased secreting beta-defensin108 (vBD108) of blue fox is speculated be related to asthenospermia. To clarify this idea, the mRNA expression of vBD108 in testis and epididymis of blue foxes with asthenospermia were detected and compared to the healthy one. The antibody was prepared and analyzed by immunohistochemistry.
RESULTS
The vBD108 in testis and epididymis was found both in blue fox with asthenospermia and healthy group by the method of immunohistochemistry. The expression of vBD108 mRNA in testes (P < 0.05) and epididymal corpus (P < 0.0001) in asthenospermia group was lower than that in healthy group.
CONCLUSIONS
These results suggested that vBD108 deficiency may related to blue fox asthenospermia. Meanwhile, the study on the blue fox vBD108 provides a hopeful direction to explore the pathogenesis of blue fox asthenospermia in the future.
背景
繁殖力对于养殖蓝狐（北极狐）很重要，在中国一些养殖场，弱精子症已成为一个问题。弱精子症的一个关键症状是精子活力下降。推测蓝狐β-防御素108（vBD108）分泌减少与弱精子症有关。为了阐明这一观点，检测并比较了弱精子症蓝狐与健康蓝狐睾丸和附睾中vBD108的mRNA表达。制备了抗体并通过免疫组织化学进行分析。
结果
通过免疫组织化学方法，在弱精子症蓝狐组和健康组的睾丸和附睾中均发现了vBD108。弱精子症组睾丸（P＜0.05）和附睾体（P＜0.0001）中vBD108 mRNA的表达低于健康组。
结论
这些结果表明，vBD108缺乏可能与蓝狐弱精子症有关。同时，对蓝狐vBD108的研究为未来探索蓝狐弱精子症的发病机制提供了一个有希望的方向。
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