Utiger Julia M, Glas Michael, Levis Anja, Prazak Josef, Haenggi Matthias
Department of Intensive Care Medicine, Bern University Hospital, Inselspital, University of Bern, Bern, Switzerland.
Department of Anaesthesiology and Pain Medicine, Bern University Hospital, Inselspital, University of Bern, Bern, Switzerland.
BMC Res Notes. 2021 Jan 7;14(1):14. doi: 10.1186/s13104-020-05438-y.
Standard rodent sepsis models as cecal ligation and puncture models (CLP) or cecal ligation and incision models (CLI) are frequently not suited experiments, mainly because they lack surgical repair, and they are difficult to control for severity. The colon ascendens stent peritonitis model (CASP) overcomes some of these limitations.
Here we present our modification of the rodent CASP model, where severity of sepsis can be controlled by timing of surgical repair and treatment, and by diameter of the stent. Further, basic hemodynamic monitoring (blood pressure and heart rate) and frequent blood sampling can be achieved, which might guide further treatment.
标准的啮齿动物脓毒症模型，如盲肠结扎穿刺模型（CLP）或盲肠结扎切开模型（CLI），常常不适用于实验，主要是因为它们缺乏手术修复，且严重程度难以控制。升结肠支架腹膜炎模型（CASP）克服了其中一些局限性。
在此，我们展示了对啮齿动物CASP模型的改良，脓毒症的严重程度可通过手术修复和治疗的时机以及支架直径来控制。此外，还可进行基本的血流动力学监测（血压和心率）以及频繁的血液采样，这可能会为进一步治疗提供指导。
