Bilaloğlu Mustafa Hilkat, Tarhan Ömer Rıdvan, Zihni İsmail, Şirin Mümtaz Cem
General Surgery Clinic, Bayburt State Hospital, Bayburt-Türkiye.
Department of General Surgery, Suleyman Demirel University Faculty of Medicine, Isparta-Türkiye.
Ulus Travma Acil Cerrahi Derg. 2024 Jan;30(12):852-860. doi: 10.14744/tjtes.2024.57634.
This study aims to examine the effect of Leukocyte-Rich Platelet-Rich Plasma (LR-PRP) on bacterial translocation in an experimental peritonitis model in rats. Secondary peritonitis occurs due to the loss of integrity in the mucosal barrier of the gastrointestinal system, resulting from contamination of the peritoneal cavity by microorganisms. LR-PRP has been shown to have positive anti-infectious, immunomodulatory, and angiogenetic effects.
Twenty-seven Wistar-Albino rats were divided into three groups: Sham, Control, and Experimental. Laparotomy was performed on the rats under anesthesia, and the cecum was isolated. No procedure was performed on the Sham group. The cecums of the rats in the Control and Experimental groups were punctured twice within 5 minutes using an 18-gauge needle. The blood product of each rat in the Experimental group was prepared for autologous use as LR-PRP and administered intraperitoneally. The abdomens of rats in all groups were closed after 8 minutes. After 8 hours, the rats were sacrificed, and tissue and blood samples were collected. Inflammatory parameters (TNF-α, IL-1, and IL-6) and blood cultures were analyzed from the blood samples. Cultures were also performed on liver, spleen, and mesenteric lymph node tissue samples.
Liver tissue culture growth was not detected in rats in the sham group. It was detected in 6 rats in the control group, and in 1 rat in the experimental group. Mesenteric lymph node tissue culture growth was detected in 2 rats in the sham group, in 7 in the control group and in 1 in the experimental group. Blood culture growth was not detected in rats in the sham group, but detected in 8 rats in the control group, and 3 in the experimental group. In terms of liver tissue culture, mesenteric lymph node tissue culture, and blood culture; a significant relationship was statistically observed between the control and experimental groups (p=0.049, p=0.008, p=0.015, respectively). It was infered that a statistically significant relationship in the mean TNF-alpha, IL-1, IL-6 values was not seen between the control and experimental groups (p=0.999, p=0.999, p=0.590, respectively).
LR-PRP's ability to suppress bacterial translocation was statistically significant in liver tissue culture, mesenteric lymph node tissue culture, and blood culture when comparing the Control and Experimental groups. LR-PRP was found to be effective in preventing bacterial translocation without suppressing inflammation and exhibited antimicrobial properties as supported by the literature.
本研究旨在探讨富含白细胞的富血小板血浆（LR-PRP）对大鼠实验性腹膜炎模型中细菌移位的影响。继发性腹膜炎是由于胃肠道系统黏膜屏障完整性丧失所致，这是由微生物污染腹膜腔引起的。LR-PRP已被证明具有积极的抗感染、免疫调节和血管生成作用。
将27只Wistar白化大鼠分为三组：假手术组、对照组和实验组。在麻醉下对大鼠进行剖腹手术，分离出盲肠。假手术组不进行任何操作。对照组和实验组的大鼠在5分钟内用18号针头对盲肠穿刺两次。将实验组每只大鼠的血液制品制备成自体LR-PRP并腹腔内给药。8分钟后关闭所有组大鼠的腹部。8小时后，处死大鼠，收集组织和血液样本。对血液样本分析炎症参数（TNF-α、IL-1和IL-6）并进行血培养。对肝脏组织、脾脏组织和肠系膜淋巴结组织样本也进行培养。
假手术组大鼠肝脏组织培养未检测到生长。对照组6只大鼠检测到生长，实验组1只大鼠检测到生长。假手术组2只大鼠肠系膜淋巴结组织培养检测到生长，对照组7只，实验组1只。假手术组大鼠血培养未检测到生长，但对照组8只大鼠检测到生长，实验组3只检测到生长。就肝脏组织培养、肠系膜淋巴结组织培养和血培养而言，对照组和实验组之间在统计学上观察到显著关系（分别为p = 0.049、p = 0.008、p = 0.015）。推断对照组和实验组之间在平均TNF-α、IL-1、IL-6值方面未观察到统计学上的显著关系（分别为p = 0.999、p = 0.999、p = 0.590）。
在比较对照组和实验组时，LR-PRP抑制细菌移位的能力在肝脏组织培养、肠系膜淋巴结组织培养和血培养中具有统计学意义。发现LR-PRP在不抑制炎症的情况下有效预防细菌移位，并如文献所支持的那样具有抗菌特性。
