[八种核酸染料的安全性研究] （你提供的原文似乎不完整，推测是这个意思，你可根据实际情况调整）
[Safety of eight nucleic acid dyeson].
作者信息
Yu Wen, An Lin, Chen Yiwen, Ren Xiu, Luo Haipeng, Liu Na, Cui Shenghui, Li Jingyun
机构信息
National Institutes for Food and Drug Control, Beijing 100050, China.
出版信息
Wei Sheng Yan Jiu. 2020 Nov;49(6):938-948. doi: 10.19813/j.cnki.weishengyanjiu.2020.06.011.
OBJECTIVE
To study the safety of eight nucleic acid dyes: EB, GelGreen, GoldView, GeneGreen, SYBRGreen I, GelRed, SYBRSafe and SYBRGold.
METHODS
Salmonell atyphimurium TA97, TA98, TA100 and TA102 involved in the minimum inhibitory concentration(MIC) of the 8 nucleic acid dyes by the Broth dilution, which follows Clinical and Laboratory Standards Institute. The experiment tests the reverse mutation of bacteria with the 8 nucleic acid dyes, which follows GB 15193. 4-2014.
RESULTS
EB and its metabolites show significant mutagenicity on TA97, TA98, TA100 and TA102. SYBRGold and its metabolites do not show mutagenicity on TA97, TA98, TA100 and TA102. The other six dyes show varying mutagenicity on TA97, TA98 and TA102, meanwhile show no mutagenicity on TA100.
CONCLUSION
This research shows that, except EB, the other dyes show no mutagenicity under working concentration, but show varying mutagenicity under high concentration. SYBRSafe and SYBRGreenI are highly toxic, and the operators must have high precautions when making diluted solution of them.
目的
研究8种核酸染料（EB、GelGreen、GoldView、GeneGreen、SYBRGreen I、GelRed、SYBRSafe和SYBRGold）的安全性。
方法
采用肉汤稀释法，按照美国临床和实验室标准协会的方法，测定8种核酸染料对鼠伤寒沙门氏菌TA97、TA98、TA100和TA102的最低抑菌浓度（MIC）。按照GB 15193.4-2014的方法，用这8种核酸染料检测细菌的回复突变。
结果
EB及其代谢产物对TA97、TA98、TA100和TA102显示出显著的致突变性。SYBRGold及其代谢产物对TA97、TA98、TA100和TA102未显示出致突变性。其他6种染料对TA97、TA98和TA102显示出不同程度的致突变性，而对TA100未显示出致突变性。
结论
本研究表明，除EB外，其他染料在工作浓度下未显示出致突变性，但在高浓度下显示出不同程度的致突变性。SYBRSafe和SYBRGreenI毒性较高，操作人员在配制其稀释溶液时必须高度谨慎。
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