Department of Clinical Pharmacy, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, School of Pharmacy, Nanjing, Jiangsu Province, 211198, People's Republic of China.
School of Pharmacy, Nanjing Medical University, Nanjing, 211166, Jiangsu Province, China.
Biomed Microdevices. 2021 Jan 8;23(1):5. doi: 10.1007/s10544-020-00538-9.
Bone marrow-derived mesenchymal stem cells (BMSCs) have been considered a promising therapeutic approach to cardiovascular disease. This study intends to compare the effect of BMSCs through a standard active cardiac support device (ASD) and intravenous injection on global myocardial injury induced by isoproterenol. BMSCs were cultured in vitro, and the transplanted cells were labeled with a fluorescent dye CM-Dil. Isoproterenol (ISO) was injected into the rats; 2 weeks later, the labeled cells were transplanted into ISO-induced heart-jury rats through the tail vein or ASD device for 5 days. The rats were sacrificed on the first day, the third day, and the fifth day after transplantation to observe the distribution of cells in the myocardium by fluorescence microscopy. The hemodynamic indexes of the left ventricle were measured before sacrificing. H&E staining and Masson's trichrome staining were used to evaluate the cardiac histopathology. In the ASD groups, after 3 days of transplantation, there were a large number of BMSCs on the epicardial surface, and after 5 days of transplantation, BMSCs were widely distributed in the ventricular muscle. But in the intravenous injection group, there were no labeled-BMSCs distributed. In the ASD + BMSCs-three days treated group and ASD + BMSCs -five days-treated group, left ventricular systolic pressure (LVSP), the maximum rate of left ventricular pressure rise (+dP/dt), the maximum rate of left ventricular pressure decline (-dP/dt) increased compared with model group and intravenous injection group (P < 0.05). By giving BMSCs through ASD device, cells can rapidly and widely distribute in the myocardium and significantly improve heart function.
骨髓间充质干细胞（BMSCs）已被认为是一种有前途的治疗心血管疾病的方法。本研究旨在比较 BMSCs 通过标准主动心脏支持装置（ASD）和静脉注射对异丙肾上腺素诱导的整体心肌损伤的作用。BMSCs 在体外培养，并使用荧光染料 CM-Dil 标记移植细胞。将异丙肾上腺素（ISO）注射到大鼠体内；2 周后，通过尾静脉或 ASD 装置将标记的细胞移植到 ISO 诱导的心肌损伤大鼠体内，持续 5 天。移植后第一天、第三天和第五天处死大鼠，通过荧光显微镜观察细胞在心肌中的分布。在处死前测量左心室的血流动力学指标。使用 H&E 染色和 Masson 三色染色评估心脏组织病理学。在 ASD 组中，移植后 3 天，心外膜表面有大量 BMSCs，移植后 5 天，BMSCs 在心室肌中广泛分布。但在静脉注射组中，没有分布标记的 BMSCs。在 ASD+BMSCs-3 天治疗组和 ASD+BMSCs-5 天治疗组中，与模型组和静脉注射组相比，左心室收缩压（LVSP）、左心室压力上升最大速率（+dP/dt）和左心室压力下降最大速率（-dP/dt）均增加（P<0.05）。通过 ASD 装置给予 BMSCs，细胞可以快速广泛地分布在心肌中，显著改善心功能。
