Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, School of Pharmacy, Xuzhou Medical University, Xuzhou, 221002, P. R. China.
Org Biomol Chem. 2021 Jan 28;19(4):911-919. doi: 10.1039/d0ob02253e. Epub 2021 Jan 8.
Hydrogen polysulfides (HS, n > 1), which are important reactive sulfur species, play crucial roles in HS-related bioactivities, including antioxidation, cytoprotection, activation of ion channels, transcription factor functions and tumour suppression. Monitoring HSin vivo is of significant interest for exploring the physiological roles of HS and the exact mechanisms of HS-related diseases. Herein, we conceive a novel near-infrared fluorescent probe, NIR-CPS, that is used to detect HS in living cells and in vivo. With the advantages of high sensitivity, good selectivity and a remarkably large Stokes shift (100 nm), NIR-CPS was successfully applied in visualizing HS in living cells and mice. More importantly, NIR-CPS monitored HS stimulated by lipopolysaccharide in tumour-bearing mice. These results demonstrate that the NIR-CPS probe is a potentially powerful tool for the detection of HSin vivo, thus providing a valuable approach in HS-related medical research.
氢多硫化物（HS，n＞1）是重要的活性硫物种，在 HS 相关生物活性中发挥着关键作用，包括抗氧化、细胞保护、离子通道激活、转录因子功能和肿瘤抑制。监测 HS 体内水平对于探索 HS 的生理作用以及 HS 相关疾病的确切机制具有重要意义。在此，我们设计了一种新型的近红外荧光探针 NIR-CPS，用于检测活细胞和体内的 HS。NIR-CPS 具有高灵敏度、良好选择性和显著斯托克斯位移（100nm）的优点，成功应用于活细胞和小鼠中 HS 的可视化。更重要的是，NIR-CPS 监测了荷瘤小鼠中脂多糖刺激的 HS。这些结果表明，NIR-CPS 探针是体内 HS 检测的一种潜在有力工具，从而为 HS 相关医学研究提供了一种有价值的方法。
