†Key Laboratory of Coastal Environmental Processes and Ecological Remediation, The Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
‡The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, China.
Anal Chem. 2015 Apr 7;87(7):3631-8. doi: 10.1021/ac5044237. Epub 2015 Mar 17.
Hydrogen polysulfides (H2Sn, n > 1), derived from hydrogen sulfide (H2S), have attracted increasing attention in biochemical research, which may perform as the actual signaling molecules during cell signaling processes. Because of the closed biological and chemical relationship between H2S and H2Sn, it is of great value to develop sensitive and specific techniques to distinguish the intracellular level of H2Sn. To improve the understanding of the physiological and pathological roles played by H2Sn, we now develop a specific fluorescent probe Mito-ss for capturing H2Sn in cells and in vivo. When triggered by H2Sn, Mito-ss replies a turn-on fluorescence signal and exhibits a higher selectivity toward H2Sn than other abundant competing biothiols, such as glutathione, cysteine and H2S. The probe Mito-ss can also be applied to visual H2Sn in living cells, as well as in vivo, providing a potentially powerful approach for probing H2Sn in biological systems.
氢多硫化物（H2Sn，n＞1）来源于硫化氢（H2S），在生化研究中受到越来越多的关注，它们可能在细胞信号转导过程中作为实际的信号分子发挥作用。由于 H2S 和 H2Sn 之间的封闭的生物和化学关系，开发灵敏和特异的技术来区分细胞内 H2Sn 的水平具有重要价值。为了提高对 H2Sn 发挥的生理和病理作用的理解，我们现在开发了一种用于在细胞内和体内捕获 H2Sn 的特异性荧光探针 Mito-ss。当被 H2Sn 触发时，Mito-ss 回复一个开启的荧光信号，并且对 H2Sn 的选择性高于其他丰富的竞争生物硫醇，如谷胱甘肽、半胱氨酸和 H2S。该探针 Mito-ss 还可用于在活细胞以及体内可视化 H2Sn，为在生物系统中探测 H2Sn 提供了一种潜在的强大方法。
