State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Heilongtan, Northern suburb, Kunming, 650201, Yunnan, People's Republic of China.
Key Laboratory of Agro-Biodiversity and Pest Management of Ministry of Education, Yunnan Agricultural University, Kunming, 650201, Yunnan, People's Republic of China.
Protoplasma. 2021 Jul;258(4):743-752. doi: 10.1007/s00709-020-01591-0. Epub 2021 Jan 8.
Rice blast disease is one of the most common rice diseases worldwide. It is essential to improve disease resistance through environment-friendly methods, while maintaining yield and quality parameters. In this study, jasmonic acid (JA), a plant hormone with anti-fungal activity, was obtained, at both low (100 μmol/L) and high (400 μmol/L) concentrations in rice leaves, before, during, and after infection, respectively. JA could inhibit germination and appressorium formation of rice blast spores in a dose-dependent manner. A total of 400-μmol/L JA treatment significantly enhanced cell viability and endogenous JA level in rice leaves. Furthermore, rice leaves inoculated with Magnaporthe oryzae and sprayed with JA 72 h post-inoculation showed the maximum symptom relief and the highest endogenous JA production among all treatment approaches. The expressions of defense-related genes, OsPR10a and OsAOS2, were highly up-regulated in response to JA, whereas OsEDS1 was down-regulated. Hence, we revealed that exogenous JA could activate JA signaling to effectively control the symptoms of rice blast.
稻瘟病是全球范围内最常见的水稻病害之一。通过环保的方法提高抗病性，同时保持产量和质量参数至关重要。在这项研究中，我们在水稻叶片中分别于感染前后，获得了低浓度（100 μmol/L）和高浓度（400 μmol/L）的茉莉酸（JA），一种具有抗真菌活性的植物激素。JA 可以以剂量依赖的方式抑制稻瘟病菌孢子的萌发和附着胞的形成。400 μmol/L 的 JA 处理显著提高了水稻叶片的细胞活力和内源 JA 水平。此外，在接种 Magnaporthe oryzae 后 72 小时用 JA 喷雾处理的水稻叶片，在所有处理方法中表现出最大的症状缓解和最高的内源 JA 产生。防御相关基因 OsPR10a 和 OsAOS2 的表达水平在 JA 处理下显著上调，而 OsEDS1 的表达水平则下调。因此，我们揭示了外源 JA 可以激活 JA 信号通路，从而有效地控制稻瘟病的症状。
