Sirhindi Geetika, Mir Mudaser Ahmad, Abd-Allah Elsayed Fathi, Ahmad Parvaiz, Gucel Salih
Department of Botany, Punjabi University Patiala, India.
Plant Production Department, College of Food and Agricultural Sciences, King Saud University Riyadh, Saudi Arabia.
Front Plant Sci. 2016 May 12;7:591. doi: 10.3389/fpls.2016.00591. eCollection 2016.
In present study, we evaluated the effects of Jasmonic acid (JA) on physio-biochemical attributes, antioxidant enzyme activity, and gene expression in soybean (Glycine max L.) plants subjected to nickel (Ni) stress. Ni stress decreases the shoot and root length and chlorophyll content by 37.23, 38.31, and 39.21%, respectively, over the control. However, application of JA was found to improve the chlorophyll content and length of shoot and root of Ni-fed seedlings. Plants supplemented with JA restores the chlorophyll fluorescence, which was disturbed by Ni stress. The present study demonstrated increase in proline, glycinebetaine, total protein, and total soluble sugar (TSS) by 33.09, 51.26, 22.58, and 49.15%, respectively, under Ni toxicity over the control. Addition of JA to Ni stressed plants further enhanced the above parameters. Ni stress increases hydrogen peroxide (H2O2) by 68.49%, lipid peroxidation (MDA) by 50.57% and NADPH oxidase by 50.92% over the control. Supplementation of JA minimizes the accumulation of H2O2, MDA, and NADPH oxidase, which helps in stabilization of biomolecules. The activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) increases by 40.04, 28.22, 48.53, and 56.79%, respectively, over the control in Ni treated seedlings and further enhancement in the antioxidant activity was observed by the application of JA. Ni treated soybean seedlings showed increase in expression of Fe-SOD by 77.62, CAT by 15.25, POD by 58.33, and APX by 80.58% over the control. Nevertheless, application of JA further enhanced the expression of the above genes in the present study. Our results signified that Ni stress caused negative impacts on soybean seedlings, but, co-application of JA facilitate the seedlings to combat the detrimental effects of Ni through enhanced osmolytes, activity of antioxidant enzymes and gene expression.
在本研究中，我们评估了茉莉酸（JA）对遭受镍（Ni）胁迫的大豆（Glycine max L.）植株的生理生化特性、抗氧化酶活性和基因表达的影响。与对照相比，镍胁迫使地上部和根部长度以及叶绿素含量分别降低了37.23%、38.31%和39.21%。然而，发现施用茉莉酸可提高镍处理幼苗的叶绿素含量以及地上部和根部的长度。补充茉莉酸的植株恢复了受镍胁迫干扰的叶绿素荧光。本研究表明，在镍毒性条件下，脯氨酸、甘氨酸甜菜碱、总蛋白和总可溶性糖（TSS）分别比对照增加了33.09%、51.26%、22.58%和49.15%。向镍胁迫植株添加茉莉酸进一步提高了上述参数。与对照相比，镍胁迫使过氧化氢（H2O2）增加了68.49%，脂质过氧化（MDA）增加了50.57%，NADPH氧化酶增加了50.92%。补充茉莉酸可使H2O2、MDA和NADPH氧化酶的积累最小化，这有助于生物分子的稳定。在镍处理的幼苗中，超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）的活性分别比对照增加了40.04%、28.22%、48.53%和56.79%，并且通过施用茉莉酸观察到抗氧化活性进一步增强。与对照相比，镍处理的大豆幼苗中Fe-SOD的表达增加了77.62%，CAT增加了15.25%，POD增加了58.33%，APX增加了80.58%。然而，在本研究中，施用茉莉酸进一步增强了上述基因的表达。我们的结果表明，镍胁迫对大豆幼苗产生负面影响，但是，茉莉酸的共同施用通过增强渗透调节物质、抗氧化酶活性和基因表达，促进幼苗对抗镍的有害影响。
