打破规则：NEIL2 调控中的蛋白质塑造
Breaking the Rules: Protein Sculpting in NEIL2 Regulation.
机构信息
Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
出版信息
Structure. 2021 Jan 7;29(1):1-2. doi: 10.1016/j.str.2020.12.011.
Abstract
The canonical DNA glycosylase role is global base damage repair but includes functions in epigenetic gene regulation, immune response modulation, replication, and transcription. In this issue of Structure, Eckenroth et al. (2020) present the NEIL2 glycosylase structure. Its catalytic domain flexibility differentiates it from most other glycosylases and suggests novel regulatory mechanisms.
摘要
规范的 DNA 糖苷酶的作用是进行全局碱基损伤修复，但它也具有调节表观遗传基因、免疫反应、复制和转录等功能。在本期的《结构》杂志中，Eckenroth 等人（2020 年）展示了 NEIL2 糖苷酶的结构。其催化结构域的灵活性使其与大多数其他糖苷酶区分开来，并提出了新的调控机制。
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Nucleic Acids Res. 2021 Jan 11;49(1):221-243. doi: 10.1093/nar/gkaa1120.
2
Tautomerization-dependent recognition and excision of oxidation damage in base-excision DNA repair.碱基切除DNA修复中氧化损伤的互变异构依赖性识别与切除
Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):7792-7. doi: 10.1073/pnas.1604591113. Epub 2016 Jun 27.
3
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Nat Struct Mol Biol. 2016 Feb;23(2):116-124. doi: 10.1038/nsmb.3151. Epub 2016 Jan 11.
4
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5
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6
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