Chen Yigang, Huang Pei, Chen Hui, Wang Sen, Wang Hao, Guo Jian, Zhang Xiaonong, Zhang Shaoxiang, Yan Jun, Xia Jiazeng, Xu Zekuan
Department of Pathology, Nanjing Jinling Hospital, Nanjing University School of Medicine, Jiangsu 210000, People's Republic of China.
State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
ACS Biomater Sci Eng. 2018 Dec 10;4(12):4095-4103. doi: 10.1021/acsbiomaterials.8b00957. Epub 2018 Oct 15.
Little attention has been paid to the biocompatibility and biological effects of zinc as a material. Here, we therefore investigated the biocompatibility and anti-inflammatory and collagen-promoting effects of pure zinc material in the colorectum. Our in vitro results indicated that zinc toxicity and concentration were closely related. Low concentrations of zinc ions and pure zinc material extract had only minor effects on the viability of primary rectal mucosal epithelial cells; however, cytotoxicity was observed at concentrations greater than 0.017 μg/μL and 60%, respectively. In vivo experiments demonstrated that zinc pins degraded slowly in the colorectum (their volume decreasing by approximately 7.79% over 1 month) and did not cause serious adverse reactions. Pure zinc material was found to inhibit acute inflammation through increased expression of ENA-78 and F4/80. Moreover, zinc material heightened expression of collagen and VEGF, factors conducive to wound healing, in surrounding colorectal tissues. These preliminary results suggest that zinc shows great promise as an implant material for medical applications involving colorectal surgery.
锌作为一种材料，其生物相容性和生物学效应一直未受到太多关注。因此，我们在此研究了纯锌材料在结肠直肠中的生物相容性、抗炎作用和促进胶原蛋白生成的作用。我们的体外实验结果表明，锌的毒性与浓度密切相关。低浓度的锌离子和纯锌材料提取物对原代直肠黏膜上皮细胞的活力仅有轻微影响；然而，当浓度分别大于0.017μg/μL和60%时，观察到了细胞毒性。体内实验表明，锌钉在结肠直肠中降解缓慢（1个月内其体积减少约7.79%），且未引起严重不良反应。发现纯锌材料通过增加ENA-78和F4/80的表达来抑制急性炎症。此外，锌材料提高了结肠直肠周围组织中胶原蛋白和VEGF（有利于伤口愈合的因子）的表达。这些初步结果表明，锌作为涉及结肠直肠手术的医学应用植入材料具有很大的潜力。
