Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, Higashi 1-1-1, Tsukuba, Ibaraki, 305-8566, Japan.
Department of Periodontology, Osaka University Graduate School of Dentistry, 1-8 Yamada-oka, Suita, Osaka, 565-0851, Japan.
Sci Rep. 2021 Jan 8;11(1):228. doi: 10.1038/s41598-020-80546-0.
PM2.5 has been correlated with risk factors for various diseases and infections. It promotes tissue injury by direct effects of particle components. However, effects of PM2.5 on cells have not been fully investigated. Recently, we developed a novel imaging technology, scanning electron-assisted dielectric-impedance microscopy (SE-ADM), which enables observation of various biological specimens in aqueous solution. In this study, we successfully observed PM2.5 incorporated into living mammalian cells in culture media. Our system directly revealed the process of PM2.5 aggregation in the cells at a nanometre resolution. Further, we found that the PM2.5 aggregates in the intact cells were surrounded by intracellular membrane-like structures of low-density in the SE-ADM images. Moreover, the PM2.5 aggregates were shown by confocal Raman microscopy to be located inside the cells rather than on the cell surface. We expect our method to be applicable to the observation of various nanoparticles inside cells in culture media.
PM2.5 与各种疾病和感染的风险因素有关。它通过颗粒成分的直接作用促进组织损伤。然而，PM2.5 对细胞的影响尚未得到充分研究。最近，我们开发了一种新型成像技术，扫描电子辅助介电阻抗显微镜（SE-ADM），它可以观察水相中的各种生物样本。在这项研究中，我们成功地观察到 PM2.5 掺入培养介质中的活哺乳动物细胞。我们的系统以纳米分辨率直接揭示了 PM2.5 在细胞内聚集的过程。此外，我们发现，在 SE-ADM 图像中，完整细胞内的 PM2.5 聚集体被低密度的细胞内类似膜结构包围。此外，共聚焦拉曼显微镜显示 PM2.5 聚集体位于细胞内，而不是细胞表面。我们期望我们的方法适用于观察培养介质中细胞内各种纳米颗粒。
