Baysal Asli, Saygin Hasan, Ustabasi Gul Sirin
Health Services Vocational School of Higher Education, T.C. Istanbul Aydin University, Sefakoy Kucukcekmece, 34295, Istanbul, Turkey.
Application and Research Center for Advanced Studies, T.C. Istanbul Aydin University, Sefakoy Kucukcekmece, 34295, Istanbul, Turkey.
Environ Monit Assess. 2017 Dec 21;190(1):34. doi: 10.1007/s10661-017-6408-2.
A significant knowledge gap in nanotechnology is the absence of standardized protocols for examining and comparison the effect of metal oxide nanoparticles on different environment media. Despite the large number of studies on ecotoxicity of nanoparticles, most of them disregard the particles physicochemical transformation under real exposure conditions and interaction with different environmental components like air, soil, water, etc. While one of the main exposure ways is inhalation and/or atmosphere for human and environment, there is no investigation between airborne particulates and nanoparticles. In this study, some metal oxide nanoparticle (ZnO and TiO) transformation and behavior in PM2.5 air particulate media were examined and evaluated by the influence on nanoparticle physicochemical properties (size, surface charge, surface functionalization) and on bacterium (Gram-positive Bacillus subtilis, Staphylococcus aureus/Gram-negative Escherichia coli, Pseudomonas aeruginosa bacteria) by testing in various concentrations of PM2.5 airborne particulate media to contribute to their environmental hazard and risk assessment in atmosphere. PM2.5 airborne particulate media affected their toxicity and physicochemical properties when compared the results obtained in controlled conditions. ZnO and TiO surfaces were functionalized mainly with sulfoxide groups in PM2.5 air particulates. In addition, tested particles were not observed to be toxic in controlled conditions. However, these were observed inhibition in PM2.5 airborne particulates media by the exposure concentration. These observations and dependence of the bacteria viability ratio explain the importance of particulate matter-nanoparticle interaction.
纳米技术中一个显著的知识空白是缺乏用于检测和比较金属氧化物纳米颗粒对不同环境介质影响的标准化方案。尽管对纳米颗粒的生态毒性进行了大量研究，但大多数研究都忽略了颗粒在实际暴露条件下的物理化学转变以及与空气、土壤、水等不同环境成分的相互作用。虽然人类和环境的主要暴露途径之一是吸入和/或大气，但尚未对空气中的颗粒物与纳米颗粒之间进行研究。在本研究中，通过测试在各种浓度的PM2.5空气颗粒物介质中对纳米颗粒物理化学性质（尺寸、表面电荷、表面功能化）和对细菌（革兰氏阳性枯草芽孢杆菌、金黄色葡萄球菌/革兰氏阴性大肠杆菌、铜绿假单胞菌）的影响，研究并评估了一些金属氧化物纳米颗粒（ZnO和TiO）在PM2.5空气颗粒物介质中的转变和行为，以有助于其在大气中的环境危害和风险评估。与在受控条件下获得的结果相比，PM2.5空气颗粒物介质影响了它们的毒性和物理化学性质。在PM2.5空气颗粒物中，ZnO和TiO表面主要被亚砜基团功能化。此外，在受控条件下未观察到测试颗粒具有毒性。然而，通过暴露浓度观察到这些颗粒在PM2.5空气颗粒物介质中受到抑制。这些观察结果以及细菌存活率的依赖性解释了颗粒物与纳米颗粒相互作用的重要性。
