骨骼肌肌联蛋白的巨大多肽：盐酸胍中的沉降平衡
Giant polypeptides of skeletal muscle titin: sedimentation equilibrium in guanidine hydrochloride.
作者信息
Kurzban G P, Wang K
机构信息
Clayton Foundation Biochemical Institute, Department of Chemistry, Austin, Texas.
出版信息
Biochem Biophys Res Commun. 1988 Feb 15;150(3):1155-61. doi: 10.1016/0006-291x(88)90750-4.
PMID:3342063
Abstract
Titin has been purified from rabbit skeletal myofibrils in guanidine hydrochloride by gel filtration on Sephacryl S-1000. The sedimentation coefficients of denatured titin in 6 M guanidine hydrochloride are highly concentration dependent and follow the equation: 1/S = 0.241 + 0.302 A280. Equilibrium sedimentation studies indicate that the average molecular weight of titin polypeptides is 2.4 to 2.6 X 10(6).
摘要
肌联蛋白已通过在盐酸胍中对兔骨骼肌肌原纤维进行凝胶过滤，利用Sephacryl S - 1000进行纯化。变性肌联蛋白在6 M盐酸胍中的沉降系数高度依赖于浓度，并遵循以下方程：1/S = 0.241 + 0.302 A280。平衡沉降研究表明，肌联蛋白多肽的平均分子量为2.4至2.6×10⁶。
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