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不同酶处理对兔骨骼肌肌原纤维中肌联蛋白片段释放的影响。一种800 kDa肌联蛋白多肽的纯化。

Effects of different enzymic treatments on the release of titin fragments from rabbit skeletal myofibrils. Purification of an 800 kDa titin polypeptide.

作者信息

Astier C, Labbé J P, Roustan C, Benyamin Y

机构信息

Centre de Recherches de Biochimie Macromoléculaire LP 9008 (C.N.R.S.), Université Montpellier I, France.

出版信息

Biochem J. 1993 Mar 15;290 ( Pt 3)(Pt 3):731-4. doi: 10.1042/bj2900731.

DOI:10.1042/bj2900731
PMID:8457201
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1132341/
Abstract

In myofibrils, titin (also called connectin) molecules span from Z line to M line and constitute a third filament system containing an elastic domain in the I band. This giant protein is particularly sensitive to proteolysis in situ. Treatment of rabbit skeletal myofibrils with exogenous proteinases induces a release of titin fragments, which are detected in the soluble myofibrillar fraction. The cleavage of titin occurs at specific points localized at the proximity of Z line and could lead to a concomitant release of alpha-actinin.

摘要

在肌原纤维中，肌联蛋白（也称为连接蛋白）分子从Z线延伸至M线，构成了第三个细丝系统，该系统在I带含有一个弹性结构域。这种巨大的蛋白质在原位对蛋白水解特别敏感。用外源性蛋白酶处理兔骨骼肌原纤维会诱导肌联蛋白片段的释放，这些片段可在可溶性肌原纤维组分中检测到。肌联蛋白的切割发生在Z线附近的特定位置，可能会导致α-辅肌动蛋白的同时释放。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0619/1132341/04c295a8986e/biochemj00115-0103-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0619/1132341/b3f44a21f833/biochemj00115-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0619/1132341/6cc614e81839/biochemj00115-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0619/1132341/1eb3f733059c/biochemj00115-0103-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0619/1132341/2ce511dd25ba/biochemj00115-0103-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0619/1132341/0f4131f2623e/biochemj00115-0103-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0619/1132341/04c295a8986e/biochemj00115-0103-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0619/1132341/b3f44a21f833/biochemj00115-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0619/1132341/6cc614e81839/biochemj00115-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0619/1132341/1eb3f733059c/biochemj00115-0103-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0619/1132341/2ce511dd25ba/biochemj00115-0103-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0619/1132341/0f4131f2623e/biochemj00115-0103-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0619/1132341/04c295a8986e/biochemj00115-0103-e.jpg

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本文引用的文献

1
Connectin, an elastic protein of muscle. Identification of "titin" with connectin.连接蛋白，一种肌肉中的弹性蛋白。“肌联蛋白”与连接蛋白的鉴定。
J Biochem. 1981 Mar;89(3):701-9. doi: 10.1093/oxfordjournals.jbchem.a133249.
2
Molecular size and shape of beta-connectin, an elastic protein of striated muscle.横纹肌弹性蛋白β-连接蛋白的分子大小和形状
J Biochem. 1984 May;95(5):1423-33. doi: 10.1093/oxfordjournals.jbchem.a134750.
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Stability of two Ca2+-dependent neutral proteinases and their specific inhibitor during post-mortem storage of rabbit skeletal muscle.
来自心肌、平滑肌和骨骼肌的α-辅肌动蛋白同工型形成肌动蛋白丝的极性阵列。
J Cell Biol. 2000 May 1;149(3):635-46. doi: 10.1083/jcb.149.3.635.
4
Alpha actinin-CapZ, an anchoring complex for thin filaments in Z-line.α辅肌动蛋白-帽蛋白，一种位于Z线的细肌丝锚定复合体。
J Muscle Res Cell Motil. 1999 Feb;20(2):187-97. doi: 10.1023/a:1005489319058.
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Fish muscle cytoskeleton integrity is not dependent on intact thin filaments.鱼类肌肉细胞骨架的完整性并不依赖于完整的细肌丝。
J Muscle Res Cell Motil. 1997 Jun;18(3):285-94. doi: 10.1023/a:1018665924412.
J Sci Food Agric. 1983 Nov;34(11):1241-50. doi: 10.1002/jsfa.2740341113.
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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
5
Connectin filaments link thick filaments and Z lines in frog skeletal muscle as revealed by immunoelectron microscopy.免疫电子显微镜显示，连接丝在青蛙骨骼肌中连接粗肌丝和Z线。
J Cell Biol. 1985 Dec;101(6):2167-72. doi: 10.1083/jcb.101.6.2167.
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A physiological role for titin and nebulin in skeletal muscle.肌联蛋白和伴肌动蛋白在骨骼肌中的生理作用。
Nature. 1986;323(6084):160-4. doi: 10.1038/323160a0.
7
The positional stability of thick filaments in activated skeletal muscle depends on sarcomere length: evidence for the role of titin filaments.激活的骨骼肌中粗肌丝的位置稳定性取决于肌节长度：肌联蛋白丝作用的证据。
J Cell Biol. 1987 Nov;105(5):2217-23. doi: 10.1083/jcb.105.5.2217.
8
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J Cell Biol. 1989 Nov;109(5):2169-76. doi: 10.1083/jcb.109.5.2169.