（S，E）-4-氨基-5-氟戊-2-烯酸使γ-氨基丁酸转氨酶失活的机制
Mechanism of inactivation of gamma-aminobutyric acid aminotransferase by (S,E)-4-amino-5-fluoropent-2-enoic acid.
作者信息
Silverman R B, George C
机构信息
Department of Chemistry, Northwestern University, Evanston, Illinois 60208.
出版信息
Biochem Biophys Res Commun. 1988 Feb 15;150(3):942-6. doi: 10.1016/0006-291x(88)90720-6.
Evidence for an enamine mechanism of inactivation of pig brain gamma-aminobutyric acid (GABA) aminotransferase by (S,E)-4-amino-5-fluoropent-2-enoic acid is presented. apo-GABA aminotransferase reconstituted with [3H]pyridoxal 5'-phosphate is inactivated by (S,E)-4-amino-5-fluoropent-2-enoic acid and the pH is raised to 12. All of the radioactivity is released from the enzyme as an adduct of the cofactor; no [3H]pyridoxamine 5'-phosphate is generated.
本文提供了关于(S,E)-4-氨基-5-氟戊-2-烯酸使猪脑γ-氨基丁酸（GABA）转氨酶失活的烯胺机制的证据。用[3H]吡哆醛5'-磷酸重构的脱辅基GABA转氨酶被(S,E)-4-氨基-5-氟戊-2-烯酸失活，并且pH升高到12。所有放射性作为辅因子的加合物从酶中释放出来；没有生成[3H]吡哆胺5'-磷酸。
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