Graduate School of Beijing University of Chinese Medicine, Beijing, 100029, China.
Cardiovascular Disease Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China.
Chin J Integr Med. 2021 Apr;27(4):259-264. doi: 10.1007/s11655-020-3479-7. Epub 2021 Jan 9.
To reveal the underlying relationships between Chinese medicine (CM) syndromes and ultrafiltration (UF) in the treatment of heart failure based on a metabonomic approach.
Seventeen acute decompensated heart failure (ADHF) patients were enrolled, and their CM syndromes before and after UF were collected. In addition, their venous plasma collected before and after UF was used for liquid chromatographmass spectrometer-based metabonomic analysis. Both reversed phase liquid chromatography and hydrophilic interaction liquid chromatography were used to analyze the plasma samples. Partial least-squares to latent structure-discriminant analyses were used for data analysis.
An obvious difference was observed pre- and post-treatment. A total of 17 potential biomarkers associating with alterd syndromes with UF including hypoxanthine, 1-methylhistidine, phytosphingosine, O-decanoyl-R-carnitine, etc. were screened out, showing a significant change after UF. The major adjusted metabolic pathways were purine metabolism, histidine metabolism, leucine and isoleucine metabolism, arginine and proline metabolism, carnitine shuttle, sphingolipid metabolism and phospholipid metabolism.
Metabonomic approach is a useful tool to identify potential biomarkers of altered syndromes link to UF and could provide a theoretical basis for further research on the therapeutic mechanism of UF combined with CM.
基于代谢组学方法揭示心力衰竭超滤治疗中中医证候与超滤之间的潜在关系。
纳入 17 例急性失代偿性心力衰竭（ADHF）患者，采集超滤前后的中医证候，同时采集超滤前后的静脉血浆进行基于液质联用的代谢组学分析。采用反相液相色谱和亲水相互作用液相色谱分析血浆样本。采用偏最小二乘-判别分析进行数据分析。
治疗前后观察到明显差异。共筛选出 17 种与超滤相关的证候改变的潜在生物标志物，包括次黄嘌呤、1-甲基组氨酸、植物鞘氨醇、O-癸酰基-R-肉碱等，超滤后均显示出显著变化。主要调整代谢途径为嘌呤代谢、组氨酸代谢、亮氨酸和异亮氨酸代谢、精氨酸和脯氨酸代谢、肉碱穿梭、鞘脂代谢和磷脂代谢。
代谢组学方法是识别与超滤相关的证候改变的潜在生物标志物的有效工具，可为超滤联合中医治疗机制的进一步研究提供理论依据。
