翻译：eIF2α 磷酸化调控翻译的起源：裂殖酵母全基因组翻译谱研究的新见解。
Origin of translational control by eIF2α phosphorylation: insights from genome-wide translational profiling studies in fission yeast.
机构信息
Molecular Cellular and Developmental Biology Program, Division of Biology, Kansas State University, Manhattan, KS, 66506, USA.
Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima, 739-8530, Japan.
出版信息
Curr Genet. 2021 Jun;67(3):359-368. doi: 10.1007/s00294-020-01149-w. Epub 2021 Jan 9.
Abstract
During amino acid limitation, the protein kinase Gcn2 phosphorylates the α subunit of eIF2, thereby regulating mRNA translation. In yeast Saccharomyces cerevisiae and mammals, eIF2α phosphorylation regulates translation of related transcription factors Gcn4 and Atf4 through upstream open reading frames (uORFs) to activate transcription genome wide. However, mammals encode three more eIF2α kinases activated by distinct stimuli. Did the translational control system involving eIF2α phosphorylation evolve from so simple (as found in yeast S. cerevisiae) to complex (as found in humans)? Recent genome-wide translational profiling studies of amino acid starvation response in the fission yeast Schizosaccharomyces pombe provide an unexpected answer to this question.
摘要
在氨基酸缺乏的情况下，蛋白激酶 Gcn2 使 eIF2 的α亚基磷酸化，从而调节 mRNA 的翻译。在酵母酿酒酵母和哺乳动物中，eIF2α 磷酸化通过上游开放阅读框 (uORFs) 调节相关转录因子 Gcn4 和 Atf4 的翻译，从而激活全基因组的转录。然而，哺乳动物编码三种更多的 eIF2α 激酶，它们被不同的刺激激活。涉及 eIF2α 磷酸化的翻译控制系统是否从如此简单（如在酵母酿酒酵母中发现）进化到复杂（如在人类中发现）？最近对裂殖酵母 Schizosaccharomyces pombe 中氨基酸饥饿反应的全基因组翻译分析研究为这个问题提供了一个意想不到的答案。
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