Shanghai Engineering Research Center of Food Safety, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Institute of Agricultural Information and Economic, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Food Chem. 2021 Jun 1;346:128952. doi: 10.1016/j.foodchem.2020.128952. Epub 2020 Dec 26.
Practical application of proanthocyanidins (PAs) as antioxidants is limited because of their hard-to-maintained activities during the processes and storage and in severe gastrointestinal environments. To overcome this challenge, we have developed an easy and green method to encapsulate PAs based on casein-maltodextrin Maillard conjugates. The current work entails the systematic study on the antioxidative potentials of fabricated casein-maltodextrin-PAs nanoparticles (CMPNs). In vitro antioxidant activities of CMPNs remained well during storage in 28 days and treatments under 40-80 °C. In vivo Caenorhabditis elegans (C. elegans) model further showed that the CMPNs could prolong the lifespan of nematodes and protected nematodes from oxidative stress and heat shock. Analyses of intracellular superoxide dismutase and catalase activities also confirmed the existence of an antioxidant protective effect. Besides, in vitro release test showed that the encapsulation enhanced the bioaccessibility of PAs. These results have important implications for the development of novel antioxidants in nutraceutical industries.
原花青素（PAs）作为抗氧化剂的实际应用受到限制，因为它们在加工和储存过程中以及在严重的胃肠道环境中难以保持活性。为了克服这一挑战，我们开发了一种基于酪蛋白-麦芽糊精美拉德轭合物来包封 PAs 的简单绿色方法。目前的工作涉及对所制备的酪蛋白-麦芽糊精-PAs 纳米颗粒（CMPNs）的抗氧化潜力进行系统研究。在 28 天的储存和 40-80°C 的处理过程中，CMPNs 的体外抗氧化活性保持良好。体内秀丽隐杆线虫（C. elegans）模型进一步表明，CMPNs 可以延长线虫的寿命，并保护线虫免受氧化应激和热休克的影响。细胞内超氧化物歧化酶和过氧化氢酶活性的分析也证实了抗氧化保护作用的存在。此外，体外释放试验表明，包封提高了 PAs 的生物利用度。这些结果对营养保健品行业中新型抗氧化剂的开发具有重要意义。
