Centro de Referencia para Lactobacilos (CERELA-CCT NOA Sur-CONICET), Chacabuco 145, T4000ILC, San Miguel de Tucumán, Tucumán, Argentina; Centro Científico Tecnológico NOA Sur - CONICET, Crisóstomo Álvarez 722, T4000ILC, San Miguel de Tucumán, Tucumán, Argentina.
Centro Científico Tecnológico NOA Sur - CONICET, Crisóstomo Álvarez 722, T4000ILC, San Miguel de Tucumán, Tucumán, Argentina; Universidad Nacional de Tucumán, Ayacucho 491, T4000INI, San Miguel de Tucumán, Tucumán, Argentina.
Res Vet Sci. 2021 Mar;135:27-35. doi: 10.1016/j.rvsc.2020.12.020. Epub 2020 Dec 29.
The intake of antinutritional factors produce impairment on the intestinal digestive function, impeding the efficient use of nutrients. Probiotics could be useful in poultry breeding to prevent negative effects of antinutritional factors, like the dietary lectins soybean agglutinin (SBA) and wheat germ agglutinin (WGA). Therefore, this investigation aimed to verify that SBA and wheat, which contains WGA, exert harmful effects on the intestinal mucosa and the digestive system of young poultry, and determine if the administration of probiotics able to capture lectins could counteract their effects. The trials performed demonstrated that a mixture of Bifidobacterium infantis CRL 1395, Enterococcus faecium LET 301, Lactobacillus salivarius LET 201, L. reuteri LET 210, and Propionibacterium acidipropionici LET 103, strains with ex vivo ability to interfere with the interaction of lectins and epithelial cells, has no negative effect on young chickens health. Middle levels of SBA, as well as wheat as a source of WGA, resulted in lower activities of intestinal and brush border enzymes and alterations in the integrity and morphological parameters of the chicks jejunal mucosa. The bacteria blend increased the activity of several digestive enzymes and the intestinal maturation marker alkaline phosphatase in birds fed with a conventional diet. Besides, it partially countered the deleterious effects of increased content of SBA, as well as the negative effect of a dietary source of WGA, on digestive enzymes activity and intestinal mucosa integrity. The results highlight the capability of multifunctional bacterial mixtures to protect the digestive system of avian against residual dietary lectins.
抗营养因子的摄入会损害肠道消化功能，从而影响营养物质的有效利用。益生菌可用于家禽养殖，以防止抗营养因子（如大豆凝集素[SBA]和麦胚凝集素[WGA]）的负面作用。因此，本研究旨在验证 SBA 和含有 WGA 的小麦是否会对幼禽的肠道黏膜和消化系统造成损害，并确定是否可以通过添加能够捕获凝集素的益生菌来抵消其影响。试验结果表明，双歧杆菌 CRL 1395、屎肠球菌 LET 301、唾液乳杆菌 LET 201、雷特氏乳杆菌 LET 210 和丙酸杆菌 LET 103 的混合物（具有体外干扰凝集素与上皮细胞相互作用的能力）对幼鸡的健康没有负面影响。中水平的 SBA 和作为 WGA 来源的小麦会降低肠道和刷状缘酶的活性，并改变小鸡空肠黏膜的完整性和形态参数。在饲喂常规饮食的禽类中，细菌混合物增加了几种消化酶和肠道成熟标志物碱性磷酸酶的活性。此外，它部分抵消了 SBA 含量增加以及 WGA 饮食来源对消化酶活性和肠道黏膜完整性的负面影响。这些结果突出了多功能细菌混合物保护禽类消化系统免受残留日粮凝集素侵害的能力。
