Gravato Carlos, Abe Flávia Renata, de Oliveira Danielle Palma, Soares Amadeu M V M, Domingues Inês
Faculdade de Ciências & CESAM, Universidade de Lisboa, Campo Grande, Lisboa, Portugal.
School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Methods Mol Biol. 2021;2240:119-124. doi: 10.1007/978-1-0716-1091-6_10.
Acetylcholinesterase (AChE) is a useful biomarker for organophosphate and carbamate pesticides exposure. The inhibition of this enzyme has been associated with neurotoxicity and alterations at higher levels of biological organization, such as behavior and development impairments. In this chapter, we describe the methodologies for analyses of AChE activity in pools of 96 h of embryos of zebrafish (Danio rerio) using a spectrophotometric method adapted to 96-well microtiter plates.
乙酰胆碱酯酶（AChE）是有机磷和氨基甲酸酯类农药暴露的一种有用生物标志物。该酶的抑制与神经毒性以及更高生物组织水平的改变有关，如行为和发育障碍。在本章中，我们描述了使用适用于96孔微量滴定板的分光光度法分析斑马鱼（Danio rerio）96小时胚胎池中的AChE活性的方法。
