Heuschling P, De Paermentier F, van den Bosch de Aguilar P
Université Catholique de Louvain, Laboratoire de Biologie Cellulaire, Belgium.
Brain Res. 1988 Jan 1;466(1):9-17. doi: 10.1016/0165-3806(88)90081-8.
Cortex, hippocampus, septum and striatum of day 18 rat embryos were grafted to several brain regions of young adult rats which had been lesioned in the chosen area 4 days earlier. Thirty days after transplantation, the grafts were fixed and morphometrically analysed under light microscope. The volumes, neuronal densities and total number of neurons of the transplants were compared. Each graft survived best when transplanted to its original region. Good survival was also achieved by heterotopic grafts between regions that are anatomically related. Striatal grafts showed reasonable survival only when transplanted to their original site. In a second series of experiences, the neurons from the same embryonic brain regions were cultured in a defined medium, to which was added tissue extracts from the lesioned regions of the adult brain. The neuronal survival was estimated. The in vitro results are closely related to those obtained in vivo. This experimental evidence agrees with the theory of the existence of a retrograde transport of NGF from the hippocampus to the septum, sustaining the survival of the latter. On the other hand, our results demonstrate the existence of other unidentified neurotrophic factors in the central nervous system which differ from one region to another.
将18日龄大鼠胚胎的皮质、海马、隔区和纹状体移植到4天前在选定区域受过损伤的成年幼鼠的几个脑区。移植30天后，将移植物固定并在光学显微镜下进行形态计量分析。比较移植物的体积、神经元密度和神经元总数。每个移植物移植到其原始区域时存活最好。在解剖学相关区域之间进行的异位移植也能实现良好的存活。纹状体移植物只有移植到其原始部位时才显示出合理的存活。在第二系列实验中，将来自相同胚胎脑区的神经元在特定培养基中培养，并向其中添加成年脑损伤区域的组织提取物。估计神经元的存活率。体外结果与体内获得的结果密切相关。这一实验证据与NGF从海马向隔区逆行运输以维持后者存活的理论相符。另一方面，我们的结果表明中枢神经系统中存在其他未确定的神经营养因子，这些因子在不同区域有所不同。
