Department of Microbiology & Immunology, McGill University, Montreal, Quebec H3G 1Y6, Canada.
Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada.
Trends Biochem Sci. 2021 May;46(5):378-390. doi: 10.1016/j.tibs.2020.12.007. Epub 2021 Jan 7.
Virion assembly is an important step in the life cycle of all viruses. For viruses of the Flavivirus genus, a group of enveloped positive-sense RNA viruses, the assembly step represents one of the least understood processes in the viral life cycle. While assembly is primarily driven by the viral structural proteins, recent studies suggest that several nonstructural proteins also play key roles in coordinating the assembly and packaging of the viral genome. This review focuses on describing recent advances in our understanding of flavivirus virion assembly, including the intermolecular interactions between the viral structural (capsid) and nonstructural proteins (NS2A and NS2B-NS3), host factors, as well as features of the viral genomic RNA required for efficient flavivirus virion assembly.
病毒粒子组装是所有病毒生命周期中的一个重要步骤。对于黄病毒属的病毒，即一组包膜正链 RNA 病毒，组装步骤是病毒生命周期中了解最少的过程之一。虽然组装主要由病毒结构蛋白驱动，但最近的研究表明，几种非结构蛋白也在协调病毒基因组的组装和包装中发挥关键作用。本综述重点描述了我们对黄病毒粒子组装的理解的最新进展，包括病毒结构（衣壳）和非结构蛋白（NS2A 和 NS2B-NS3）之间的分子间相互作用、宿主因子以及有效组装黄病毒粒子所需的病毒基因组 RNA 的特征。
