Li Kefeng, Liu Anli, Zong Wenhao, Dai Lulu, Liu Yang, Luo Renping, Ge Shulin, Dong Guijun
College of Sports and Health, Shandong Sport University, Jinan 250102, China.
Department of Acupuncture, Rizhao Hospital of Traditional Chinese Medicine, Rizhao 276800, China.
Saudi J Biol Sci. 2021 Jan;28(1):40-49. doi: 10.1016/j.sjbs.2020.08.027. Epub 2020 Aug 21.
Lipopolysaccharides (LPSs) released by gut microbiota are correlated with the pathophysiology of osteoarthritis (OA). Exercise remodels the composition of gut microbiota. The present study investigated the hypothesis that wheel-running exercise prevents knee OA induced by high-fat diet (HFD) via reducing LPS from intestinal microorganisms. Male C57BL/6 J mice were treated with sedentary or wheel-running exercise, standard diet (13.5% kcal) or HFD (60% kcal), berberine or not according to their grouping. Knee OA severity, blood and synovial fluid LPS, cecal microbiota, and TLR4 and MMP-13 expression levels were determined. Our findings reveal that HFD treatment decreased gut microbial diversity. Increase in endotoxin-producing bacteria, decrease in gut barrier-protecting bacteria, high LPS levels in the blood and synovial fluid, high TLR4 and MMP-13 expression levels, and severe cartilage degeneration were observed. By contrast, voluntary wheel running caused high gut microbial diversity. The gut microbiota were reshaped, LPS levels in the blood and synovial fluid and TLR4 and MMP-13 expression levels were low, and cartilage degeneration was ameliorated. Berberine treatment reduced LPS levels in the samples, but decreased the diversity of intestinal flora with similar changes to that caused by HFD. In conclusion, unlike taking drugs, exercising can remodel gut microbial ecosystems, reduce the circulating levels of LPS, and thereby contribute to the relief of chronic inflammation and OA. Our findings showed that moderate exercise is a potential therapeutic approach for preventing and treating obesity-related OA.
肠道微生物群释放的脂多糖（LPS）与骨关节炎（OA）的病理生理学相关。运动可重塑肠道微生物群的组成。本研究调查了以下假设：跑步运动通过减少肠道微生物产生的LPS来预防高脂饮食（HFD）诱导的膝骨关节炎。雄性C57BL/6 J小鼠根据分组接受久坐或跑步运动、标准饮食（13.5%千卡）或HFD（60%千卡）、黄连素治疗或不治疗。测定了膝骨关节炎严重程度、血液和滑液中的LPS、盲肠微生物群以及TLR4和MMP-13表达水平。我们的研究结果表明，HFD治疗降低了肠道微生物多样性。观察到产内毒素细菌增加、肠道屏障保护细菌减少、血液和滑液中LPS水平升高、TLR4和MMP-13表达水平升高以及严重的软骨退变。相比之下，自愿跑步导致肠道微生物多样性增加。肠道微生物群得到重塑，血液和滑液中的LPS水平以及TLR4和MMP-13表达水平较低，软骨退变得到改善。黄连素治疗降低了样本中的LPS水平，但降低了肠道菌群的多样性，其变化与HFD引起的变化相似。总之，与服药不同，运动可以重塑肠道微生物生态系统，降低LPS的循环水平，从而有助于缓解慢性炎症和骨关节炎。我们的研究结果表明，适度运动是预防和治疗肥胖相关骨关节炎的一种潜在治疗方法。
