Ali Daoud, Alarifi Saud, Pandian Arjun
Department of Zoology, College of Science King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Department of Biotechnology, PRIST Deemed University, Thanjavur 613403, Tamil Nadu, India.
Saudi J Biol Sci. 2021 Jan;28(1):353-359. doi: 10.1016/j.sjbs.2020.10.013. Epub 2020 Oct 15.
(Linn.) Wettst. commonly known as waterhyssop, Brahmi plant, traditionally used for memory enhancement, nerve tonic, epilepsy, central nervous system (CNS), antidepressant, anxiety, blood pressure and antioxidant activities. Due to pharmaceutical demands its lost natural habitat. At this juncture we describe a resourceful protocol for micropropagation of water hyssop plant. Surface sterilized leaf and nodal explants were inoculated on basal MS semi-solid medium added with PGRs; auxins, cytokinins. Highest calli formation from leaf explants was obtained on NAA (2.5 mg) and showed (94.22%) accompanied via 2,4-D showed (2.5 mg; 82.43%), maximum calli formation in nodal explants was obtained on 2,4-D showed (2.5 mg; 71.14%) followed by NAA (2.5 mg) showed (62.15%), in internodes explants uppermost calli formation was obtained from 2,4-D showed (2.5 mg; 65.21%) followed by NAA (2.5 mg) showed (52.14%). The maximum somatic embryogenic callus, calli induction and formation (84%) was observed on 2,4-D + KIN (2.0 + 1.5 mg) amended solid medium. Uppermost shoot formation was observed in combination of IAA + BAP (1.0 + 1.0 mg) showed (78.54%) shoot formation followed by IBA (2.0 mg) alone showed (75.37%). The maximum shoot elongation was noticed from NAA + BAP (3.0 + 3.0 mg) with 21.21 cm followed by NAA (2.0 mg) showed (15.22 cm) although, chief root formation was obtained from IBA (2.0 mg) with 83.75% root formation along higher number of roots (47.43%) per shoot. Followed by IAA (2.0 mg) showed root induction (73.43%) and no of roots (38.54%) per shoot. In hardening under pot condition plants survivability (100%) was observed under glass house conditions, the present PTC techniques is extremely significant to gratifying its natural conservation.
（林奈）韦特施泰因，通常被称为水薄荷、婆罗门参，传统上用于增强记忆力、作为神经滋补剂、治疗癫痫、作用于中枢神经系统（CNS）、抗抑郁、缓解焦虑、调节血压以及具有抗氧化活性。由于医药需求，其天然栖息地已丧失。在此关头，我们描述了一种水薄荷植物微繁殖的实用方案。将表面灭菌的叶片和节间外植体接种在添加了植物生长调节剂（PGRs）的基础MS半固体培养基上；植物生长素、细胞分裂素。在添加萘乙酸（NAA，2.5毫克）的培养基上，叶片外植体形成愈伤组织的比例最高，为94.22%，添加2,4 -二氯苯氧乙酸（2,4 - D，2.5毫克）时形成愈伤组织的比例为82.43%；在节间外植体中，添加2,4 - D（2.5毫克）时愈伤组织形成比例最高，为71.14%，其次是添加萘乙酸（NAA，2.5毫克）时，比例为62.15%；在茎节外植体中，添加2,4 - D（2.5毫克）时愈伤组织形成比例最高，为65.21%，其次是添加萘乙酸（NAA，2.5毫克）时，比例为52.14%。在添加2,4 - D + 激动素（KIN，2.0 + 1.5毫克）的改良固体培养基上，观察到最大的体细胞胚性愈伤组织、愈伤组织诱导和形成比例（84%）。在吲哚乙酸（IAA） + 苄氨基嘌呤（BAP，1.0 + 1.0毫克）组合的培养基上，芽形成比例最高，为78.54%，其次是单独使用吲哚丁酸（IBA，2.0毫克）时，芽形成比例为75.37%。在添加萘乙酸（NAA） + 苄氨基嘌呤（BAP，3.0 + 3.0毫克）的培养基上，芽伸长最长，为21.21厘米，其次是添加萘乙酸（NAA，2.0毫克）时，芽伸长为15.22厘米；然而，主根形成则是在吲哚丁酸（IBA，2.0毫克）的培养基上，生根率为83.75%，每株芽的生根数较多（47.43%）。其次是添加吲哚乙酸（IAA，2.0毫克）时，生根诱导率为73.43%，每株芽的生根数为38.54%。在盆栽条件下炼苗时，在温室条件下观察到植株存活率为100%，目前的植物组织培养技术对于满足其自然保护具有极其重要的意义。
