Institute of Vegetables and Flowers, Chongqing Academy of Agricultural Sciences, Chongqing, 400000, China.
Sci Rep. 2023 Sep 7;13(1):14785. doi: 10.1038/s41598-023-42001-8.
The Heuchera genus, a member of the Saxifragaceae family, encompasses a wide array of varieties and hybrids, serving both traditional medicinal and ornamental purposes. However, a significant knowledge gap persists in achieving efficient mass propagation of diverse Heuchera cultivars creating a substantial market void. To address this, our study focuses on expedited seedling regeneration by investigating leaf cutting and tissue culture techniques to offer novel insights to cultivators. Herein, we successfully rooted thirteen distinct cultivars from the Heuchera and Heucherella (Heuchera × Tiarella) genera through cutting. Moreover, in vitro culture experiments led to the successful induction of calli and shoots from petiole samples. Notably, variations in measured parameters were observed across cultivars in both cutting and tissue culture methodologies. When petiole explants were exposed to cytokinin 6-benzylaminopurine (BA) at concentrations of 0.5, 1.0, and 2.0 mg/L along with auxin α-naphthaleneacetic acid (NAA) at 0.5 mg/L, shoots were produced either directly or indirectly during the primary culture. Exposure to darkness and the application of 2,4-dichlorophenoxyacetic acid (2,4-D) did not promote shoot formation but were beneficial for callus stimulation. Interestingly, a negative correlation was observed between the ease of initiating cutting recovery and inducting tissue culture regeneration, suggesting that cultivars that easily regenerate through cutting might encounter difficulties during induction by tissue culture. In light of these findings, we devised a streamlined and effective protocol for rapid Heuchera propagation. This protocol involves micropropagation, directly acquiring adventitious shoots from primary cultures supplemented by cutting-based propagation methods.
玉簪属（Heuchera）是虎耳草科（Saxifragaceae）的一个成员，包含了广泛的品种和杂交种，既具有传统的药用价值，也有观赏价值。然而，在实现多样化玉簪属品种的高效大规模繁殖方面，仍然存在着显著的知识空白，这造成了巨大的市场缺口。为了解决这个问题，我们的研究重点是通过叶片切割和组织培养技术来加速幼苗再生，为种植者提供新的见解。在此，我们通过切割成功地从玉簪属和玉簪杂种属（Heuchera × Tiarella）中生根了十三个不同的品种。此外，体外培养实验导致从叶柄样品中成功诱导愈伤组织和芽。值得注意的是，在切割和组织培养方法中，品种之间的测量参数存在差异。当叶柄外植体暴露于浓度为 0.5、1.0 和 2.0mg/L 的细胞分裂素 6-苄基氨基嘌呤（BA）和 0.5mg/L 的生长素α-萘乙酸（NAA）时，在初代培养中，无论是直接还是间接，都会产生芽。在黑暗中暴露并应用 2,4-二氯苯氧乙酸（2,4-D）不会促进芽的形成，但有利于刺激愈伤组织。有趣的是，我们观察到，叶片切割恢复的容易程度与组织培养再生的诱导之间存在负相关，这表明通过叶片切割容易再生的品种在组织培养诱导时可能会遇到困难。有鉴于此，我们设计了一种快速玉簪属繁殖的简化有效方案。该方案涉及微繁殖，通过在初代培养中补充基于切割的繁殖方法，直接获得不定芽。
