Candelier J J, Gane P, Couillin P, Martal J, Oriol R
Unité de Recherches de Biologie Prénatale, INSERM Unité 73, Paris, France.
Cell Differ. 1988 Jan;22(2):125-34. doi: 10.1016/0045-6039(88)90024-3.
We have used monoclonal antibodies to study the changes in the expression of four kidney antigens during organogenesis in the sheep. Two of these antibodies, EE24.6 and EJ30.1, label intensely only the adult kidney, whereas the other two, EK17.1 and EJ15.1, bind to the extracellular matrix of the embryonic kidney. For EJ15.1, the staining of the extracellular matrix decreases temporarily during the second half of intrauterine life, a period during which a light staining appears in the mesangium. For the other, EK17.1, the extracellular matrix staining in the stroma gradually decreases as the embryo grows, while staining of the mesangium and the arterial intima becomes evident. With EK17.1, fibronectin is identified in the extracellular matrix of the embryonic kidney and intracellularly in the mesangial cells after these cells have colonized the glomerulus. The mesonephros staining seems to be the same as that of the metanephros. In the adult, extraglomerular vascular endothelial cells bind EK17.1, whereas intraglomerular endothelial cells do not express fibronectin, which suggests a functional difference between endothelial cells in these two localizations.
我们已使用单克隆抗体来研究绵羊器官发生过程中四种肾脏抗原表达的变化。其中两种抗体，EE24.6和EJ30.1，仅强烈标记成年肾脏，而另外两种抗体，EK17.1和EJ15.1，则与胚胎肾脏的细胞外基质结合。对于EJ15.1，在子宫内生命的后半期，细胞外基质的染色暂时减少，在此期间系膜中出现淡染色。对于另一种抗体EK17.1，随着胚胎的生长，基质中的细胞外基质染色逐渐减少，而系膜和动脉内膜的染色变得明显。使用EK17.1时，在系膜细胞定植于肾小球后，可在胚胎肾脏的细胞外基质和系膜细胞内鉴定出纤连蛋白。中肾的染色似乎与后肾相同。在成年动物中，肾小球外血管内皮细胞结合EK17.1，而肾小球内内皮细胞不表达纤连蛋白，这表明这两种定位的内皮细胞之间存在功能差异。
