Wu Xiao, Yan Ming, Lu Jiamin, Ge Xingyun, Li Yuzhi, Bian Minxia, Fu Lin, Yu Jinhua
Key Laboratory of Oral Diseases of Jiangsu Province, Institute of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing 210029, China.
Endodontic Department, School of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing 210029, China.
Stem Cells Int. 2020 Dec 24;2020:6673467. doi: 10.1155/2020/6673467. eCollection 2020.
The regeneration of bone and tooth tissues, and related cellular therapies, has attracted widespread attention. Bone marrow mesenchymal stem cells (BMSCs) are potential candidates for such regeneration. iRoot SP is a premixed bioceramic root canal sealer widely used in clinical settings. However, the effect of iRoot SP on the biological features of BMSCs has not been elucidated. In the present study, we found that 0.2 mg/ml iRoot SP conditioned medium promoted osteo/odontogenic differentiation and enhanced mineralization of BMSCs without affecting the proliferative ability. Mechanistically, the NF-B and MAPK signaling pathways were activated in SP-treated BMSCs, and differentiation was inhibited when cultured with the specific inhibitor. Taken together, these findings demonstrate that iRoot SP promotes osteo/odontogenic differentiation of BMSCs via the NF-B and MAPK signaling pathways, which could provide a new theoretical basis for clinical applications of iRoot SP and a new therapeutic target for the regeneration of bone and tooth tissue in the future.
骨组织和牙齿组织的再生以及相关的细胞治疗已引起广泛关注。骨髓间充质干细胞（BMSCs）是此类再生的潜在候选细胞。iRoot SP是一种预混生物陶瓷根管封闭剂，广泛应用于临床。然而，iRoot SP对BMSCs生物学特性的影响尚未阐明。在本研究中，我们发现0.2mg/ml iRoot SP条件培养基可促进BMSCs的成骨/成牙分化并增强其矿化能力，且不影响其增殖能力。机制上，SP处理的BMSCs中NF-κB和MAPK信号通路被激活，与特异性抑制剂共培养时分化受到抑制。综上所述，这些发现表明iRoot SP通过NF-κB和MAPK信号通路促进BMSCs的成骨/成牙分化，这可为iRoot SP的临床应用提供新的理论依据，并为未来骨组织和牙齿组织的再生提供新的治疗靶点。
