State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, Department of Conservative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, Sichuan, China.
Division of Endodontics, Department of Oral Biological & Medical Sciences, Faculty of Dentistry, The University of British Columbia, Vancouver, British Columbia, Canada.
Clin Oral Investig. 2023 Dec 22;28(1):11. doi: 10.1007/s00784-023-05402-7.
The aim of this study was to evaluate the effects of Hiflow with other two kinds of root canal sealers on the biological behavior of stem cells from the apical papilla (SCAP), the influence on inflammatory cytokines release and its antibacterial effects.
Material extracts of Hiflow, iRoot SP, and AH Plus were prepared. Then, SCAP was incubated with extracts. The effects were evaluated by CCK-8, wound healing assay, ALP staining, alizarin red staining, and qRT-PCR. Meanwhile, polymorphonuclears (PMNs) and monocytes were isolated and treated with extracts for 4 h and 24 h respectively. Cell viability was analyzed by Annexin-V/PI double staining flow cytometry. The effects on the release of cytokines were observed by ELISA. The antibacterial effects of different sealers were tested against three kinds of bacteria found in chronic apical periodontitis.
A series of results of SCAP showed that Hiflow and iRoot SP could promote cell proliferation, migration, and osteogenesis (p < 0.05). Although Hiflow was associated with greater cell apoptosis and necrosis when incubated with PMNs and monocytes (p < 0.05), it had an approximate release of anti-inflammatory cytokines with iRoot SP, which was higher than AH plus (p < 0.05). The co-culture showed that Hiflow and iRoot SP inhibited the colony formation of F. nucleatum (p < 0.05). However, both sealers had no obvious antibacterial effect on E. faecalis and P. gingivalis (p > 0.05).
In summary, Hiflow and iRoot SP both had positive biological stimulus on SCAP. Meanwhile, Hiflow showed a better induction on anti-inflammatory cytokines over the others. All the properties mentioned above and its antibacterial effect of F. nucleatum promise Hiflow a bright application prospect in endodontic uses.
References for clinical work to use BC Sealer Hiflow as a good biological root canal sealer.
本研究旨在评估 Hiflow 与另外两种根管封闭剂对根尖乳头干细胞（SCAP）生物学行为的影响，以及对炎性细胞因子释放的影响及其抗菌作用。
制备 Hiflow、iRoot SP 和 AH Plus 的材料提取物。然后，将 SCAP 与提取物孵育。通过 CCK-8、划痕愈合试验、ALP 染色、茜素红染色和 qRT-PCR 评估效果。同时，分离多形核细胞（PMN）和单核细胞，分别用提取物处理 4 h 和 24 h。通过 Annexin-V/PI 双染色流式细胞术分析细胞活力。通过 ELISA 观察对细胞因子释放的影响。测试不同封闭剂对慢性根尖周炎中发现的三种细菌的抗菌作用。
一系列 SCAP 结果表明，Hiflow 和 iRoot SP 可促进细胞增殖、迁移和成骨作用（p<0.05）。尽管 Hiflow 与 PMN 和单核细胞孵育时与更大的细胞凋亡和坏死相关（p<0.05），但与 iRoot SP 相比，其抗炎细胞因子的释放近似，高于 AH plus（p<0.05）。共培养表明 Hiflow 和 iRoot SP 抑制 F. nucleatum 的菌落形成（p<0.05）。然而，两种封闭剂对 E. faecalis 和 P. gingivalis 均无明显的抗菌作用（p>0.05）。
总之，Hiflow 和 iRoot SP 对 SCAP 均具有积极的生物学刺激作用。同时，Hiflow 在诱导抗炎细胞因子方面优于其他两者。上述所有特性及其对 F. nucleatum 的抗菌作用预示着 Hiflow 在牙髓学中的应用前景光明。
为临床工作提供使用 BC Sealer Hiflow 作为良好的生物根管封闭剂的参考。
