Putuo District People's Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Oxid Med Cell Longev. 2020 Dec 22;2020:3515609. doi: 10.1155/2020/3515609. eCollection 2020.
Schott [Shi Chang Pu in Chinese (SCP)] is a traditional Chinese medicine frequently used in the clinical treatment of dementia, amnesia, epilepsy, and other mental disorders. Previous studies have shown the potential efficacy of SCP against Alzheimer's disease (AD). Nevertheless, the active constituents and the modes of action of SCP in AD treatment have not been fully elucidated.
The aim of this study was to investigate the protective effects of SCP on abnormal proteins and clarify its molecular mechanisms in the treatment of AD by using a () model.
This study experimentally assessed the effect of SCP-Oil in CL4176 strains expressing human in muscle cells and CL2355 strains expressing human in -neurons. Western blotting, qRT-PCR, and fluorescence detection were performed to determine the oxidative stress and signaling pathways affected by SCP-Oil in nematodes.
SCP-Oil could significantly reduce the deposition of misfolded and polyQ proteins and improved serotonin sensitivity and olfactory learning skill in worms. The analysis of pharmacological action mechanism of SCP-Oil showed that its maintaining protein homeostasis is dependent on the autophagy pathway regulated partly by and genes.
Our results provide new insights to develop treatment strategy for AD by targeting autophagy, and SCP-Oil could be an alternative drug for anti-AD.
石菖蒲是一种常用于治疗痴呆、健忘、癫痫等精神障碍的中药。既往研究表明石菖蒲提取物对阿尔茨海默病（AD）具有潜在疗效，但石菖蒲治疗 AD 的活性成分及其作用机制尚未完全阐明。
本研究旨在通过 （ ）模型，探讨石菖蒲油对异常蛋白的保护作用，并阐明其治疗 AD 的分子机制。
本研究采用肌肉细胞表达人 （CL4176 株）和神经元表达人 （CL2355 株）的实验方法，评估石菖蒲油的作用。采用 Western blot、qRT-PCR 和荧光检测方法，检测石菖蒲油对线虫氧化应激和信号通路的影响。
石菖蒲油可显著减少错误折叠的 和多聚谷氨酰胺蛋白的沉积，并改善线虫的血清素敏感性和嗅觉学习能力。石菖蒲油的药理作用机制分析表明，其维持蛋白质平衡依赖于自噬途径，该途径部分受 和 基因调控。
本研究为通过靶向自噬途径开发 AD 治疗策略提供了新的思路，石菖蒲油可能是一种治疗 AD 的替代药物。
