Zhu Meiju, Zhu Hongzhu, Tan Ninghua, Zeng Guangzhi, Zeng Zhigang, Chu Hongbiao, Wang Hui, Xia Zhi, Wu Renle
School of Sports Science, Jinggangshan University, Jian 343009, Jiangxi, China.
School of Sports Science, Jinggangshan University, Jian 343009, Jiangxi, China.
J Ethnopharmacol. 2014 Dec 2;158 Pt A:431-6. doi: 10.1016/j.jep.2014.10.026. Epub 2014 Nov 5.
Acorus tatarinowii Schott (Shi Chang Pu) belongs to the family of Acoraceae. The plant is used as an important herb for prolonging life many years in traditional Chinese medicine. It is an ancient herbal tonic nutriment and can be used as anti-fatigue medicine. However, the effects of Acorus tatarinowii Schott on the endurance exercise in relation to central nervous system have not yet been clarified. In this study, the effects of Acorus tatarinowii Schott on treadmill running endurance, 5-HT concentrations, TPH2, 5-HT1B expression in the dorsal raphe of exercised rats were investigated.
Sixty adult male Sprague-Dawley rats were randomly divided into six groups: the normal group, the exercise group, the exercise and the rhizomes of Acorus tatarinowii Schott (ATS)(1mg/kg)-treated group, the exercise and ATS (10mg/kg)-treated group, the exercise and ATS (100mg/kg)-treated group, the exercise and caffeine (10mg/kg)-treated group. The effects of Acorus tatarinowii Schott on endurance exercise were determined by the time to exhaustion during treadmill exercise. The detection of 5-HT concentrations in the dorsal raphe was performed by HPLC analysis. The levels of TPH2, 5-HT1A and 5-HT1B expression were measured by western blot analysis and real-time PCR.
We found Acorus tatarinowii Schott could prolong the time to exhaustion in treadmill exercise and suppress the exercise-induced increase of 5-HT synthesis, TPH2 mRNA and protein expression and prevent the exercise-induced decrease of 5-HT1B mRNA and protein expression in the dorsal raphe. Acorus tatarinowii Schott was as effective as caffeine in prolonging the exhaustion time in treadmill running and in decreasing the exercise-induced increase of 5-HT synthesis and TPH2 mRNA and protein expression and in preventing the exercise-induced decrease of 5-HT1B mRNA and protein expression in the dorsal raphe.
The results indicated that the effects of Acorus tatarinowii Schott in inhibiting the exercise-induced synthesis of 5-HT and TPH2 expression and in preventing the exercise-induced decrease of 5-HT1B expression in the dorsal raphe might be the anti-fatigue mechanism of Acorus tatarinowii Schott.
石菖蒲属于菖蒲科植物。在传统中药中，该植物作为延年益寿的重要草药已有多年应用历史。它是一种古老的滋补营养草药，可作为抗疲劳药物。然而，石菖蒲对耐力运动的影响及其与中枢神经系统的关系尚未阐明。本研究探讨了石菖蒲对运动大鼠跑步机跑步耐力、5-羟色胺（5-HT）浓度、色氨酸羟化酶2（TPH2）以及中缝背核中5-HT1B表达的影响。
60只成年雄性Sprague-Dawley大鼠随机分为6组：正常组、运动组、运动+石菖蒲根茎（1mg/kg）治疗组、运动+石菖蒲根茎（10mg/kg）治疗组、运动+石菖蒲根茎（100mg/kg）治疗组、运动+咖啡因（10mg/kg）治疗组。通过跑步机运动至疲劳的时间来确定石菖蒲对耐力运动的影响。采用高效液相色谱分析法检测中缝背核中的5-HT浓度。通过蛋白质免疫印迹分析和实时定量聚合酶链反应检测TPH2、5-HT1A和5-HT1B的表达水平。
我们发现石菖蒲可延长跑步机运动至疲劳的时间，抑制运动诱导的5-HT合成增加、TPH2 mRNA和蛋白表达，并防止运动诱导的中缝背核中5-HT1B mRNA和蛋白表达降低。在延长跑步机跑步至疲劳时间、减少运动诱导的5-HT合成增加以及TPH2 mRNA和蛋白表达，以及防止运动诱导的中缝背核中5-HT1B mRNA和蛋白表达降低方面，石菖蒲与咖啡因效果相当。
结果表明，石菖蒲抑制运动诱导的5-HT合成和TPH2表达以及防止运动诱导的中缝背核中5-HT1B表达降低的作用可能是其抗疲劳的机制。
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