Ibrahim Ahmed, Hassan Dalia, Kelany Noura, Kotb Saber, Soliman Mahmoud
Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
Department of Animal and Poultry Hygiene, and Environmental Sanitation, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
Front Vet Sci. 2020 Dec 23;7:597751. doi: 10.3389/fvets.2020.597751. eCollection 2020.
Tilapia fish skin has demonstrated promise as a stable and practical biological dressing to be used in wound and burn management. However, the appropriate sterilization technique of the Tilapia fish skin is crucial before its clinical application. The standard sterilization technique must eliminate harmful pathogens but maintain the structural and biochemical properties that could compromise the dressing function. This study investigated and compared the efficiency of three sterilizing agents; chlorhexidine gluconate 4% (CHG), povidone iodine 10% (PVP-I), and silver nanoparticles (25 μg/mL) (AgNPs), at three different times (5, 10, and 15 min) on Tilapia fish skin based on the microbial count, histological and collagen properties. Among the sterilization procedures, AgNPs showed rapid and complete antimicrobial activity, with a 100% reduction in microbial growth of the fish skin throughout the treated times. Furthermore, AgNPs did not impair the cellular structure or collagen fibers content of the fish skin. However, CHG and PVP-I caused alterations in the collagen content. This study demonstrated that the AgNPs treatment of Tilapia fish skin provided sterile skin while preserving the histological properties and structural integrity. These findings provide an efficient and quick sterilization method suitable for Tilapia fish skin that could be adopted as a biological dressing.
罗非鱼鱼皮已被证明有望成为一种用于伤口和烧伤处理的稳定且实用的生物敷料。然而，罗非鱼鱼皮在临床应用前，合适的灭菌技术至关重要。标准灭菌技术必须消除有害病原体，但要保持可能影响敷料功能的结构和生化特性。本研究基于微生物计数、组织学和胶原蛋白特性，研究并比较了三种灭菌剂（4%葡萄糖酸氯己定（CHG）、10%聚维酮碘（PVP-I）和25μg/mL银纳米颗粒（AgNPs））在三个不同时间点（5、10和15分钟）对罗非鱼鱼皮的灭菌效果。在所有灭菌程序中，AgNPs显示出快速且完全的抗菌活性，在整个处理时间内鱼皮的微生物生长减少了100%。此外，AgNPs并未损害鱼皮的细胞结构或胶原蛋白纤维含量。然而，CHG和PVP-I导致了胶原蛋白含量的改变。本研究表明，用AgNPs处理罗非鱼鱼皮可提供无菌皮肤，同时保留组织学特性和结构完整性。这些发现提供了一种适用于罗非鱼鱼皮的高效快速灭菌方法，可将其用作生物敷料。
