López-Leyva Yoel, González-Garduño Roberto, Huerta-Bravo Maximino, Ramírez-Valverde Rodolfo, Torres-Hernández Glafiro, Arece-García Javier, López-Arellano Ma Eugenia
Posgrado en Producción Animal, Universidad Autónoma Chapingo, Estado de México, Mexico.
Unidad Regional Universitaria Sursureste, Universidad Autónoma Chapingo, Teapa, Tabasco, Mexico.
Heliyon. 2020 Dec 29;6(12):e05870. doi: 10.1016/j.heliyon.2020.e05870. eCollection 2020 Dec.
Nutritional aspects modulate the parasitological and immune response in infected sheep. The aim of the present study was to evaluate the parasitological and humoral immune response of Pelibuey sheep experimentally infected with that were fed diets with two different energy (n = 12) and protein (n = 12) levels. Twenty-four Pelibuey lambs infected with were evaluated over 12 weeks. An additional six animals were considered as a control group. Fecal egg count (FEC) was determined, in addition to packed cell volume (PCV), total plasma protein (TPP), and immunoglobulin levels (IgA, IgG, and IgM) by indirect enzyme-linked immunosorbent assay (iELISA). Data were analyzed by repeated measures over time. The lambs that received a high-energy (HighE) diet had the lowest FEC (P < 0.01) regardless of whether they received a high (HighP) or low (LowP) level of protein. The effect of energy level was also observed over time: FEC values decreased and PCV and TPP values increased. Higher immunoglobulin levels were obtained for females (P < 0.05) than males yet, overall, the energy and protein levels of the diets did not affect the response of the immunoglobulins. Over time, however, an increase in IgG and IgM was observed, whereas the IgA level remained basal.
营养因素可调节感染绵羊的寄生虫学和免疫反应。本研究的目的是评估实验性感染的佩利布埃羊的寄生虫学和体液免疫反应，这些羊被喂食两种不同能量水平（n = 12）和蛋白质水平（n = 12）的日粮。对24只感染的佩利布埃羔羊进行了为期12周的评估。另外6只动物被视为对照组。除了通过间接酶联免疫吸附测定（iELISA）测定血细胞比容（PCV）、总血浆蛋白（TPP）和免疫球蛋白水平（IgA、IgG和IgM）外，还测定了粪便虫卵计数（FEC）。对数据进行了随时间的重复测量分析。无论接受高（HighP）还是低（LowP）水平蛋白质的日粮，接受高能量（HighE）日粮的羔羊粪便虫卵计数最低（P < 0.01）。随着时间的推移也观察到了能量水平效应：粪便虫卵计数降低，血细胞比容和总血浆蛋白值升高。雌性的免疫球蛋白水平高于雄性（P < 0.05），然而总体而言，日粮的能量和蛋白质水平并未影响免疫球蛋白的反应。然而，随着时间的推移，观察到IgG和IgM增加，而IgA水平保持在基础水平。
