Colozza Gabriele
IMBA - Institute of Molecular Biotechnology.
MicroPubl Biol. 2021 Jan 5;2021. doi: 10.17912/micropub.biology.000347.
Vertebrate embryonic development is regulated by a few families of extracellular signaling molecules. embryos offer an excellent system to study the cell-cell communication signals that govern embryonic patterning. In the frog embryos, Wnt/β-catenin plays a pivotal role in regulating embryonic axis development, and modulation of the Wnt pathway is required for proper antero-posterior patterning. Recently, a novel secreted, organizer-specific Wnt inhibitor, Bighead, was identified that acts by downregulating Lrp6 plasma membrane levels. Here, I describe a method to purify biologically active Bighead protein and confirm that Bighead promotes head development
脊椎动物胚胎发育受少数几类细胞外信号分子家族调控。胚胎提供了一个绝佳的系统来研究控制胚胎模式形成的细胞间通讯信号。在青蛙胚胎中，Wnt/β-连环蛋白在调节胚胎轴发育中起关键作用，并且Wnt信号通路的调节对于正确的前后模式形成是必需的。最近，一种新的分泌型、组织者特异性Wnt抑制剂“大头”被鉴定出来，它通过下调Lrp6质膜水平发挥作用。在此，我描述一种纯化具有生物活性的“大头”蛋白的方法，并证实“大头”促进头部发育。
