肺炎克雷伯菌芳基硫酸酯酶在酪氨酰-六亚甲基二胺-β-1,3-葡聚糖及免疫吸附剂上的亲和层析

Affinity chromatography od Klebsiella arylsulfatase on tyrosyl-hexamethylenediamine-beta-1,3-glucan and immunoadsorbent.

作者信息

Murooka Y, Yim M H, Yamada T, Harada T

出版信息

Biochim Biophys Acta. 1977 Nov 23;485(1):134-40. doi: 10.1016/0005-2744(77)90200-5.

Abstract

A simple and convenient method for preparation of a highly purified arylsulfatase (EC 3.1.6.1) from Klebsiella aerogenes has been developed. Specificity of purification was achieved by using affinity chromatography on a tyrosyl-hexamethylenediamino-beta-1,3-glucan or on a solid phase immunoadsorbent. By using affinity chromatography a homogeneous enzyme was obtained with high yield. It is also proposed that the beads of curdlan type polysaccharide consisting of beta-1,3-glucan can be used as a good matrix for affinity chromatography.

摘要

已开发出一种从产气克雷伯菌制备高纯度芳基硫酸酯酶（EC 3.1.6.1）的简单便捷方法。通过在酪氨酰 - 六亚甲基二氨基 -β-1,3 - 葡聚糖或固相免疫吸附剂上进行亲和层析实现纯化的特异性。通过亲和层析以高产率获得了均一的酶。还提出由β-1,3 - 葡聚糖组成的凝胶多糖型多糖珠可作为亲和层析的良好基质。