Universidade Federal de Santa Maria, Programa de Pós-graduação em Medicina Veterinária, Santa Maria, Rio Grande do Sul, Brasil.
Universidade de Cruz Alta, Cruz Alta, Rio Grande do Sul, Brasil.
Int J Med Mushrooms. 2020;22(11):1089-1098. doi: 10.1615/IntJMedMushrooms.2020036364.
The nematocidal effect of Pleurotus ostreatus (white variety of oyster mushroom) aqueous extract (AE) was evaluated against Haemonchus contortus eggs and infective larvae (L3) in vitro and in artificially infected gerbils (Meriones unguiculatus). The chemical analyses indicated that constituents of AE are tridecanoic, tetradecanoic, linolelaidic, 9,15-octadecadienoic, and oxalic acids. P. ostreatus extract inhibited larval hatching by 100% at the concentration of 2.24 mg/mL and (50% effective concentration) EC50 of 0.73 mg/mL. In the larval development test, AE induced a larvicidal effect at the concentration of 50 mg/mL and EC50 of 17.24 mg/mL. The larval migration test revealed a reduction of 94.7% at a concentration of as low as 4 mg/mL and EC50 of 1.25 mg/mL. No significant effects of treatment with P. ostreatus AE were seen on H. contortus in the gerbil model. Thus, our results demonstrate an important nematocidal in vitro effect of P. ostreatus AE against the parasite H. contortus. However, further investigations are necessary to confirm the anthelmintic potential of P. ostreatus extract in small ruminants.
平菇（牡蛎蘑菇的白色品种）水提物（AE）对体外感染的捻转血矛线虫虫卵和幼虫（L3）以及人工感染的沙鼠（黑线毛足鼠）体内的杀线虫效果进行了评估。化学成分分析表明，AE 的成分包括十三烷酸、十四烷酸、亚油酸、9,15-十八碳二烯酸和草酸。P. ostreatus 提取物在 2.24mg/mL 的浓度下完全抑制幼虫孵化，EC50 为 0.73mg/mL。在幼虫发育试验中，AE 在 50mg/mL 的浓度下诱导幼虫致死，EC50 为 17.24mg/mL。幼虫迁移试验显示，浓度低至 4mg/mL 时，抑制率高达 94.7%，EC50 为 1.25mg/mL。在沙鼠模型中，用 P. ostreatus AE 处理捻转血矛线虫未见明显效果。因此，我们的结果表明 P. ostreatus AE 对寄生虫捻转血矛线虫具有重要的体外杀线虫作用。然而，需要进一步的研究来确认 P. ostreatus 提取物在小反刍动物中的驱虫潜力。
