The Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York; The City College of New York, CUNY.
The Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York.
J Vis Exp. 2020 Dec 22(166). doi: 10.3791/62008.
Metabolomics, the study to identify and quantify small molecules and metabolites present in an experimental sample, has emerged as an important tool to investigate the biological activities during development and diseases. Metabolomics approaches are widely employed in the study of cancer, nutrition/diet, diabetes, and other physiological and pathological conditions involving metabolic processes. An advantageous tool that aids in metabolomic profiling advocated in this paper is matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI). Its ability to detect metabolites in situ without labeling, structural modifications, or other specialized reagents, such as those used in immunostaining, makes MALDI MSI a unique tool in advancing methodologies relevant in the field of metabolomics. An appropriate sample preparation process is critical to yield optimal results and will be the focus of this paper.
代谢组学是一种研究鉴定和定量分析实验样本中小分子和代谢物的方法，已成为研究发育和疾病过程中生物活性的重要工具。代谢组学方法广泛应用于癌症、营养/饮食、糖尿病及其他涉及代谢过程的生理和病理条件的研究。本文提倡使用一种有益的代谢组学分析工具，即基质辅助激光解吸/电离质谱成像（MALDI MSI）。它具有无需标记、结构修饰或其他特殊试剂（如免疫染色中使用的试剂）原位检测代谢物的能力，这使 MALDI MSI 成为推进代谢组学领域相关方法学的独特工具。适当的样品制备过程对于获得最佳结果至关重要，这将是本文的重点。
