Engle M J, Brown D J, Dehring A F, Dooley M
Department of Pediatrics, University of Wisconsin, Madison 53792.
Exp Lung Res. 1988;14(1):121-9. doi: 10.3109/01902148809062854.
Fetal rabbit lung explants were incubated with 3.0 mM glucose and varying levels of lactate. An increase in lactate concentrations resulted in a decrease in glucose incorporation into total disaturated phosphatidylcholine. Glucose utilization for surfactant phosphatidylcholine synthesis was also reduced by approximately 35% in the presence of 5.0 mM lactate. The decreased incorporation of glucose occurred in the fatty acid portion of both total tissue disaturated phosphatidylcholine and surfactant phosphatidylcholine. The effect of lactate on glucose incorporation into pulmonary phospholipids was not affected by the presence of pyruvate in concentrations up to 500 microM. Pyruvate alone produced only a slight decrease in glucose utilization for lung phospholipid production. These data indicate that glucose and lactate are competitive substrates for late gestation surfactant phospholipid fatty acid synthesis, and that lactate is potentially a very important substrate for fetal lung development.
将胎兔肺组织外植体与3.0 mM葡萄糖和不同水平的乳酸一起孵育。乳酸浓度的增加导致葡萄糖掺入总二饱和磷脂酰胆碱的量减少。在5.0 mM乳酸存在的情况下，用于表面活性物质磷脂酰胆碱合成的葡萄糖利用率也降低了约35%。葡萄糖掺入减少发生在总组织二饱和磷脂酰胆碱和表面活性物质磷脂酰胆碱的脂肪酸部分。乳酸对葡萄糖掺入肺磷脂的影响不受浓度高达500 microM的丙酮酸存在的影响。单独的丙酮酸仅使肺磷脂生成的葡萄糖利用率略有下降。这些数据表明，葡萄糖和乳酸是妊娠后期表面活性物质磷脂脂肪酸合成的竞争性底物，并且乳酸可能是胎儿肺发育的非常重要的底物。
