College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, People's Republic of China.
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, People's Republic of China.
Environ Technol. 2022 Jun;43(15):2270-2277. doi: 10.1080/09593330.2021.1871661. Epub 2021 Jan 11.
An electrochemiluminescence (ECL) aptasensor was prepared to detect Pb with nitrogen-doped carbon quantum dots (NCQDs) as ECL materials. To prepare the working electrode, NCQDs with carboxyl groups were loaded on a glassy carbon electrode (GCE) and then Pb aptamers were covalently bound to the NCQDs to form a stable GCE/NCQDs/aptamers. On addition of Pb, the chain aptamers change to a pb G-quadruplex conformation, which lead to a large decrease in the ECL intensity. The variation of intensity and the logarithm of the Pb concentration had a good linear relationship ( = 0.998). The detection range was wide (50 pM to 387.9 nM) with a low detection limit (18.9 pM). In interference experiments, the ECL Pb aptasensor did not suffer from interference and it had good stability. The NCQDs ECL aptasensor can detect Pb quickly and accurately, and provides a fast and efficient method for detection of Pb. Compared with literatures, the Pb aptasensor has simpler preparation process, lower cost; furthermore, it is more environmentally friendly.
一种电化学发光（ECL）适体传感器被制备用来检测 Pb，其使用氮掺杂碳量子点（NCQDs）作为 ECL 材料。为了制备工作电极，将具有羧基的 NCQDs 负载在玻碳电极（GCE）上，然后将 Pb 适体共价结合到 NCQDs 上以形成稳定的 GCE/NCQDs/适体。加入 Pb 后，链适体转变为 Pb G-四链体构象，这导致 ECL 强度大幅下降。强度的变化与 Pb 浓度的对数具有良好的线性关系（r = 0.998）。检测范围很宽（50 pM 至 387.9 nM），检测限低（18.9 pM）。在干扰实验中，ECL Pb 适体传感器不受干扰，具有良好的稳定性。NCQDs ECL 适体传感器可以快速准确地检测 Pb，为 Pb 的检测提供了一种快速高效的方法。与文献相比，Pb 适体传感器具有更简单的制备过程、更低的成本；此外，它更加环保。
