College of Marine Life Science, Ocean University Of China, Qingdao, P.R. China.
Department of Biochemistry Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.
J Biomater Sci Polym Ed. 2021 Apr;32(6):813-832. doi: 10.1080/09205063.2020.1870323. Epub 2021 Feb 11.
Driven by the need to find alternatives to control infections, this work describes the development of chitosan-PMLA nanoparticulate systems as carriers for antimicrobial glycolipid. By using a simple ionic gelation method stable nanoparticle was obtained showing an encapsulation efficiency of 73.1 ± 1.3% and an average size of 217.0 ± 15.6 nm for rhamnolipids chitosan-PMLA nanoparticles (RL-CS-NPs). Glycolipid incorporation and particle size were correspondingly corroborated by FT-IR and TEM analysis. Rhamnolipids chitosan nanoparticles (RL-CS-NPs) presented the highest antimicrobial effect towards (ATCC 26695) exhibiting a minimal inhibitory concentration of 132 µg/mL and a biofilm inhibition ability of 99%. Additionally, RL-CS-NPs did not interfere with human fibroblasts viability and proliferation under the tested conditions. The results revealed that the RL-CS-NPs were able to inhibit bacterial growth showing adequate cytocompatibility and might become, after additional studies, a valuable approach to fight biofilm related-infections.
受寻找抗感染替代方法的驱动，本工作开发了壳聚糖-PMLA 纳米颗粒系统作为抗菌糖脂的载体。通过使用简单的离子凝胶化方法，获得了稳定的纳米颗粒，其囊封效率为 73.1±1.3%，平均粒径为 217.0±15.6nm 的鼠李糖脂壳聚糖纳米颗粒（RL-CS-NPs）。糖脂的包封和粒径通过傅里叶变换红外光谱（FT-IR）和透射电子显微镜（TEM）分析得到相应的证实。壳聚糖纳米颗粒（RL-CS-NPs）对（ATCC 26695）表现出最高的抗菌效果，最低抑菌浓度为 132µg/mL，生物膜抑制能力为 99%。此外，在测试条件下，RL-CS-NPs 不干扰人成纤维细胞的活力和增殖。结果表明，RL-CS-NPs 能够抑制细菌生长，具有适当的细胞相容性，经过进一步研究，可能成为一种有价值的方法来对抗与生物膜相关的感染。
