Department of Applied Chemistry, Delhi Technological University, Delhi 110042, India.
Department of Applied Chemistry, Delhi Technological University, Delhi 110042, India.
Bioelectrochemistry. 2021 Apr;138:107733. doi: 10.1016/j.bioelechem.2020.107733. Epub 2020 Dec 29.
An ultrasensitive, electrochemical biosensor has been fabricated by utilizing molybdenum disulfide (MoS) grafted reduced graphene oxide (MoS@rGO) nanohybrid as a sensing platform. Biomolecular-assisted synthetic method was adopted to synthesize MoS@rGO nanohybrid, where L-cys was used to reduce GO. The MoS@rGO nanohybrid exhibits improved electrochemical performance when it has been electrophoretically deposited onto the indium tin oxide (ITO) coated glass substrate. Further, epithelialcell adhesion moleculeantibodies (anti-EpCAM) specific to cancer biomarker has been covalently immobilized on the MoS@rGO/ITO electrodes for label-free detection of EpCAM. Electrochemical results confirm that anti-EpCAM/MoS@rGO/ITO based biosensor can detect EpCAM in the concentration range of 0.001-20 ng mL with a detection limit of 44.22 fg mL (S/N = 3). The biosensor's excellent analytical performance has been attributed to the efficient immobilization of EpCAM antibodies on the MoS@rGO surface, which results in high specificity for EpCAM antigen. The fabricated biosensor showed good selectivity, reproducibility, and stability. The successful detection of EpCAM antigen in spiked samples (human saliva, serum and urine) makes this platform an alternative method for early screening of cancer biomarker.
一种超灵敏的电化学生物传感器已被制备，其利用二硫化钼（MoS）接枝还原氧化石墨烯（MoS@rGO）纳米杂化作为传感平台。采用生物分子辅助合成方法来合成 MoS@rGO 纳米杂化，其中 L-半胱氨酸（L-cys）用于还原 GO。MoS@rGO 纳米杂化在电泳沉积到氧化铟锡（ITO）涂覆的玻璃基底上后表现出改善的电化学性能。此外，上皮细胞黏附分子抗体（anti-EpCAM）特异性地与癌症生物标志物结合，用于无标记检测 EpCAM。电化学结果证实，基于 anti-EpCAM/MoS@rGO/ITO 的生物传感器可以在 0.001-20ngmL 的浓度范围内检测 EpCAM，检测限为 44.22fgmL（S/N=3）。该生物传感器具有出色的分析性能，这归因于 EpCAM 抗体在 MoS@rGO 表面的有效固定，从而对 EpCAM 抗原具有高特异性。所制备的生物传感器表现出良好的选择性、重现性和稳定性。在添加的样品（人唾液、血清和尿液）中成功检测到 EpCAM 抗原，使得该平台成为癌症生物标志物早期筛查的替代方法。
