Department of Biochemistry, Purdue University, 175 South University St., West Lafayette, IN 47907-2063, USA; Purdue Center for Plant Biology, Purdue University, West Lafayette, IN 47907, USA.
Department of Biochemistry, Purdue University, 175 South University St., West Lafayette, IN 47907-2063, USA; Purdue Center for Plant Biology, Purdue University, West Lafayette, IN 47907, USA.
Curr Opin Biotechnol. 2021 Jun;69:112-117. doi: 10.1016/j.copbio.2020.12.014. Epub 2021 Jan 8.
Metabolic engineering is embraced as a method to sustainably enhance production of valuable phytochemicals with beneficial properties. However, successful production of these compounds in plants is not always predictable even when the pathways are fully known, frequently due to the lack of comprehensive understanding of plant metabolism as a whole, and interconnections between different primary, secondary, and hormone metabolic networks. Here, we highlight critical hidden constraints, including substrate availability, silent metabolism, and metabolic crosstalk, that impair engineering strategies. We explore how these constraints have historically been manifested in engineering attempts and propose how modern advancements will enable future strategies to overcome these impediments.
代谢工程被认为是一种可持续提高具有有益特性的有价值植物化学物质产量的方法。然而，即使完全了解途径，这些化合物在植物中的成功生产也并不总是可预测的，这通常是由于对植物代谢整体以及不同初级、次级和激素代谢网络之间的相互联系缺乏全面的理解。在这里，我们强调了关键的隐藏约束条件，包括基质可用性、沉默代谢和代谢串扰，这些条件会影响工程策略。我们探讨了这些约束条件在工程尝试中是如何表现的，并提出了现代进展将如何使未来的策略能够克服这些障碍。
