Girard P R, Wood J G, Freschi J E, Kuo J F
Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia 30322.
Dev Biol. 1988 Mar;126(1):98-107. doi: 10.1016/0012-1606(88)90243-6.
Antisera to protein kinase C (PKC) have been used to examine the presence and distribution of the enzyme in developing cerebellar cortex of postnatal rat and in cultures of rat sympathetic ganglia. In the cerebellar cortex of 2-,4-, and 6-day old rats, immunostaining was observed in areas of early-forming presynaptic terminals and growth cones. No staining was evident in the cortical proliferative zone. Beginning at 10 days postnatal, nuclear staining, not apparent at earlier stages, was prominent in Purkinje cells. In neuronal cultures of dissociated rat sympathetic ganglia, PKC was immunolocalized in cell bodies and bundles of neuronal processes. Immunoreactivity was particularly striking in growth cones of extending neurites and in axonal varicosities. These results suggest a role for PKC in neuronal growth following cell proliferation and in synaptic function. The appearance of nuclear staining in later developmental stages suggests that the enzyme may be involved in the promotion and maintenance of the differentiated state of neurons.
蛋白激酶C（PKC）抗血清已被用于检测该酶在新生大鼠发育中的小脑皮质以及大鼠交感神经节培养物中的存在和分布情况。在2日龄、4日龄和6日龄大鼠的小脑皮质中，在早期形成的突触前终末和生长锥区域观察到免疫染色。在皮质增殖区未观察到明显染色。出生后10天开始，早期未出现的核染色在浦肯野细胞中显著。在大鼠交感神经节解离的神经元培养物中，PKC免疫定位于细胞体和神经突束。免疫反应性在延伸神经突的生长锥和轴突膨体中尤为显著。这些结果表明PKC在细胞增殖后的神经元生长和突触功能中发挥作用。后期发育阶段核染色的出现表明该酶可能参与神经元分化状态的促进和维持。
