Zhang Yi, Wang Changyong, Hu Rushan, Liu Zonghua, Xue Wei
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China.
ACS Biomater Sci Eng. 2015 Mar 9;1(3):139-147. doi: 10.1021/ab500128q. Epub 2015 Feb 12.
In practical applications, biomedical materials introduced in vivo may interact with various host cells and/or biomacromolecules and alter their physiological characteristics. Biomaterial-altered cells and/or biomacromolecules may be recognized as "non-self" by the host immune system and may consequently cause further immune responses. In the present work, the gene carrier material branched polyethylenimine (1.8 kDa) (BPEI-1.8k) induced a series of alterations of human red blood cells (RBCs), such as a morphological transition from biconcave disks to spheroechinocytes, vesiculation, a size decrease, a change in surface charge from negative to positive, a cell density reduction, membrane oxidation, and PS externalization. Furthermore, BPEI-1.8k-treated RBCs caused autologous complement activation and were recognized by autologous macrophages. This implies that the biomedical material BPEI-1.8k changed the identity of the RBCs, leading to their recognition by the autologous immune system. This study provides novel insights for the biocompatibility evaluation and clinical application of biomedical materials.
在实际应用中，引入体内的生物医学材料可能会与各种宿主细胞和/或生物大分子相互作用，并改变它们的生理特性。生物材料改变的细胞和/或生物大分子可能会被宿主免疫系统识别为“非自身”，并可能因此引发进一步的免疫反应。在本研究中，基因载体材料支链聚乙烯亚胺（1.8 kDa）（BPEI-1.8k）诱导了人红细胞（RBCs）的一系列变化，如从双凹圆盘形态转变为棘状球红细胞、形成囊泡、尺寸减小、表面电荷从负变为正、细胞密度降低、膜氧化以及磷脂酰丝氨酸外翻。此外，经BPEI-1.8k处理的红细胞会引起自体补体激活，并被自体巨噬细胞识别。这意味着生物医学材料BPEI-1.8k改变了红细胞的特性，导致它们被自体免疫系统识别。本研究为生物医学材料的生物相容性评估和临床应用提供了新的见解。
