Li Chenghua, Zhong Dagen, Zhang Yi, Tuo Weiwei, Li Nan, Wang Qian, Liu Zonghua, Xue Wei
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, 510632, China.
J Mater Chem B. 2013 Apr 14;1(14):1885-1893. doi: 10.1039/c3tb00024a. Epub 2013 Mar 1.
Human red blood cells (RBCs) have high abundance in blood tissue, usually 40-50% v/v. For the in vivo administered biomedical materials in contact with blood tissue, the RBCs are the major (in most cases, undesired) targets encountered. The interaction of the biomaterials with the RBCs will unavoidably occur, affecting the structure and function of the RBCs and then the whole organism. For the clinical applications of biomaterials, this effect should be clearly elucidated since it may cause acute or chronic harm to the organism. Moreover, the RBC-based experimental results could be extended to other tissue cells to a great extent. In this study, the effect of the branched polyethyleneimines (BPEIs) as a gene carrier on the structure and function of human RBCs was studied by using different molecular weights of the BPEIs. Specifically, the RBC aggregation and lysis induced by the BPEIs were first studied; then, the structural and conformational change of hemoglobin in the presence of the BPEIs was examined by using UV-vis, fluorescence, and circular dichroism spectroscopy. Furthermore, the oxygen-carrying function of the RBCs in the presence of the BPEIs was evaluated by measuring the 2,3-diphosphoglycerate (2,3-DPG) level and oxygen-dissociation curves. The results showed that the BPEIs with certain molecular weights at certain concentrations could cause RBC aggregation and lysis, alter the structure and conformation of hemoglobin, and impair the oxygen-delivery function of the RBCs. These data provide valuable information for the molecular design and clinical applications of the BPEIs and other biomedical materials.
人类红细胞（RBCs）在血液组织中含量丰富，通常占体积的40 - 50%。对于体内施用且与血液组织接触的生物医学材料而言，红细胞是主要（在大多数情况下为不期望的）接触靶点。生物材料与红细胞之间的相互作用不可避免地会发生，影响红细胞的结构和功能，进而影响整个机体。对于生物材料的临床应用，应清楚阐明这种影响，因为它可能对机体造成急性或慢性损害。此外，基于红细胞的实验结果在很大程度上可扩展到其他组织细胞。在本研究中，通过使用不同分子量的支化聚乙烯亚胺（BPEIs）作为基因载体，研究了其对人类红细胞结构和功能的影响。具体而言，首先研究了BPEIs诱导的红细胞聚集和裂解；然后，通过紫外可见光谱、荧光光谱和圆二色光谱研究了在BPEIs存在下血红蛋白的结构和构象变化。此外，通过测量2,3 - 二磷酸甘油酸（2,3 - DPG）水平和氧解离曲线评估了在BPEIs存在下红细胞的携氧功能。结果表明，特定浓度下具有一定分子量的BPEIs可导致红细胞聚集和裂解，改变血红蛋白的结构和构象，并损害红细胞的氧输送功能。这些数据为BPEIs和其他生物医学材料的分子设计及临床应用提供了有价值的信息。
