Floros Michael C, Bortolatto Janaína F, Oliveira Osmir B, Salvador Sergio L, Narine Suresh S
Trent Centre for Biomaterials Research, Departments of Physics & Astronomy and Chemistry, Trent University, Peterborough, Ontario Canada K9J 7B8.
Department of Restorative Dentistry, Araraquara School of Dentistry, UNESP, Univ Estadual Paulista, Araraquara, SP Brazil.
ACS Biomater Sci Eng. 2016 Mar 14;2(3):336-343. doi: 10.1021/acsbiomaterials.5b00412. Epub 2016 Feb 9.
Conventional engineered polymers are strong, stable, and can interact desirably within the human body in implants and medical devices. However, bacterial colonization of medical devices and implants constructed from these materials results in numerous hospital acquired infections (HAI) and deaths each year. Polytriazole based plastics containing triazole rings and fatty acid derivatives have been synthesized from biological sources without catalysts or solvents. In this study, three amphiphilic polytriazoles with varying triazole density and hydrophilic/hydrophobic segments demonstrated broad spectrum, contact antimicrobial properties against both Gram positive and negative bacteria. SEM analysis of bacteria killed by these polymers evidence membrane damage, indicating that these polymers act by direct contact with bacterial membranes. Surface hydrophobicity of these polymers increased with increasing triazole group density, which also improved the antimicrobial efficacy. This work demonstrates that amphiphilic polytriazoles have antimicrobial properties and that future utilization of triazole modified polymers may produce self-sterilizing materials which resist bacterial contamination and formation of antibiotic resistant organisms, ideal characteristics for medically relevant biomaterials.
传统的工程聚合物强度高、稳定性好，并且在植入物和医疗设备中能在人体内产生理想的相互作用。然而，由这些材料制成的医疗设备和植入物的细菌定植每年会导致大量医院获得性感染（HAI）和死亡。含三唑环和脂肪酸衍生物的聚三唑基塑料已从生物源合成，无需催化剂或溶剂。在本研究中，三种具有不同三唑密度和亲水/疏水链段的两亲性聚三唑对革兰氏阳性和阴性细菌均表现出广谱的接触抗菌性能。对被这些聚合物杀死的细菌进行扫描电子显微镜（SEM）分析证明了膜损伤，表明这些聚合物通过与细菌膜直接接触起作用。这些聚合物的表面疏水性随着三唑基团密度的增加而增加，这也提高了抗菌效果。这项工作表明两亲性聚三唑具有抗菌性能，并且未来三唑改性聚合物的应用可能会产生抗细菌污染和抗抗生素耐药生物形成的自消毒材料，这是医学相关生物材料的理想特性。
