Sarker Bapi, Zehnder Tobias, Rath Subha N, Horch Raymund E, Kneser Ulrich, Detsch Rainer, Boccaccini Aldo R
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.
Department of Plastic and Hand Surgery, University of Erlangen-Nuremberg, 91054 Erlangen, Germany.
ACS Biomater Sci Eng. 2017 Aug 14;3(8):1730-1737. doi: 10.1021/acsbiomaterials.7b00188. Epub 2017 Jul 7.
Alginate-based hydrogels are extensively used matrices for cell encapsulation, but they need to be modified to recapitulate chemical, microstructural, and mechanical properties of the native extracellular matrix. Like other cell types, mesenchymal stem cells exhibit rounded and clustered morphologies when they are embedded in alginate hydrogels. In this study, we use covalently cross-linked oxidized alginate-gelatin hydrogels to encapsulate human adipose-derived stem cells in order to investigate cell growth, viability, and morphology during osteogenic differentiation taking advantage of the different physicochemical properties of this modified alginate-based hydrogel in comparison to those of the pristine alginate hydrogel. We investigate the effect of hydrogel compositions on stem cell behavior in 3D. Higher viability and the spreading morphology of encapsulated cells with interconnected networks were observed in high gelatin containing compositions. More filopodial protrusions from multicellular nodules were noticed during osteogenic differentiation in the hydrogels having a high amount of gelatin, confirming their suitability for cell encapsulation and bone tissue engineering applications.
基于藻酸盐的水凝胶是广泛用于细胞封装的基质，但它们需要进行改性，以重现天然细胞外基质的化学、微观结构和力学性能。与其他细胞类型一样，间充质干细胞嵌入藻酸盐水凝胶时会呈现圆形和聚集形态。在本研究中，我们使用共价交联的氧化藻酸盐 - 明胶水凝胶来封装人脂肪来源干细胞，以便利用这种改性藻酸盐水凝胶与原始藻酸盐水凝胶不同的物理化学性质，研究成骨分化过程中的细胞生长、活力和形态。我们研究了水凝胶组成对三维空间中干细胞行为的影响。在含有高浓度明胶的组合物中，观察到封装细胞具有更高的活力和相互连接网络的铺展形态。在含有大量明胶的水凝胶中，成骨分化过程中多细胞结节出现了更多丝状伪足突起，证实了它们适用于细胞封装和骨组织工程应用。
